A PHYSIOLOGICAL STUDY ON THE CHEMICAL STIMULATION OF DENTAL PULP NERVE AND AN ETIOLOGICAL STUDY ON THE PAIN WITH PULPAL DISEASES.

牙髓神经化学刺激的生理学研究和牙髓疾病疼痛的病因学研究。

• 批准号: 06671903
• 负责人: SUNAKAWA Mitsuhiro
• 金额: $ 1.41万
• 依托单位: TOKYO MEDICAL AND DENTAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671903/
• 关键词: pulpal nerve; mustard oil; inferior alveolar nerve-driven neuron; jaw muscle EMG; axon reflex; neurogenic inflammation; pulpal disease; oro-facial, head and neck pain; 機械的単一歯周組織神経線維; 頭頸部の痛み; 咀嚼筋の筋活動; 機能的単一歯周組織神経

We performed a combined study of basic animal experments on the alteration of peripheral and central nervous system responsiveness with painful pulpal stimulation and clinical research on the relationship between the oro-facial, head and neck pain and the pulpal diseases. The results obtained are as follows ;(1) A small fiber exciant and inflammatory agent, mustard oil, applied to the rat molar tooth pulp, produced an increase activities in resting EMG of the rat jaw muscles, and a significant change especially in the ipsilateral masseter muscle.(2) Chemical conditionong stimulation of mustard oil to the ipsilateral canine tooth pulp enhenced the responsiveness of the cat cervical spinal cord (C2 of C3) neurons responding to the electrical stimulation to the inferior alveolar nerve (IANDN) ; that is, a temporal increase of the excitability as well as an expansion of cutaneous receptive field was observed in the IANDNs during the post-application periods of mustard oil. These results suggested that the mustard oil pulpal stimulation could activate the central sensitization mechanisms.(3) Chemical stimulation of the tooth pulp with mustard oil increased the excitability of the single periodontal mechanoreceptive primary fiber responding to the pressure stimulation to the canine tip, and produced the sensitization, which might be mediated by the neurogenic inflammation due to the axon reflex.(4) A clinical examination of the patients suffering from the severe pain in the oro-facial, head and neck region with respect to the pulpal diseases revealed that these kinds of pain were closely related with so-called infected root canal system, and that some of them were thought to be classified into the ideopathic pain groups.These results indicated that the chemical stimulation of the tooth pulp nerve with mustard oil provided useful methods in the experimental study on the relationship between the pulpal inflammatory changes and their related pain.

本研究结合疼痛性牙髓刺激引起周围神经和中枢神经系统反应性改变的基础动物实验和口面部及头颈部疼痛与牙髓疾病关系的临床研究，对牙髓疼痛性疾病的发生机制进行了初步探讨。实验结果如下：（1）芥子油涂抹于大鼠磨牙牙髓后，可引起大鼠下颌肌静息肌电活动增加，尤其是同侧咬肌的肌电活动有明显变化。(2)芥子油刺激同侧犬牙髓，可增强猫颈髓（C2/C3）神经元对电刺激下牙槽神经（IANDN）的反应性，即在芥子油作用后，IANDN神经元兴奋性随时间延长而增强，皮肤感受野扩大。提示芥子油刺激牙髓可激活中枢致敏机制。(3)芥子油化学刺激牙髓可使牙周膜机械感受性一级纤维对压力刺激的兴奋性增加，并产生致敏作用，这种致敏作用可能通过轴突反射引起的神经源性炎症介导。(4)对因牙髓病引起的口面部及头颈部剧烈疼痛患者的临床检查表明，这些疼痛与所谓的感染根管系统密切相关，这些结果表明，芥子油化学刺激牙髓神经是一种有效的方法牙髓炎性改变与疼痛关系的实验研究。

项目成果

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Yoshihiko Tokita 等人：“脉冲 Nd：YAG 激光改变牙髓内神经的执行反应”，日本保守牙科杂志 37（秋季）。

J.W.Hu et al: Proceedings of the 7th World Congress on pain. Progress in Pain Research and Managenent. Vol.2. IASP Press, (1994)

J.W.Hu 等人：第七届世界疼痛大会论文集。

James W.Hu et al.: "Proceedings of the World Congress on Pain Progress in Pain Research and Manggement" IASP Press(Vol.2), 949 (1994)

James W.Hu 等人：“世界疼痛研究与管理进展大会论文集”IASP Press（第 2 卷），949 (1994)

H. Yamamoto et al.: "Nerve Responses to Dentinal Stimulation in Normal and Inflamed Teeth." J. Dent. Res.73. 122 (1994)

H. Yamamoto 等人：“正常和发炎牙齿对牙本质刺激的神经反应”。

Y.Tokita et al: "Infradental Nerve Activites Induced by Application of Two Kinds of Dental Temporary Cements in the Cat" Jap.J.Conserv.Dent. 37 (3). 1008-1016 (1994)

Y.Tokita 等人：“猫体内使用两种牙科临时水泥引起的牙下神经活动”Jap.J.Conserv.Dent。