The experimental study of maxillary reconstruction using Bone Morphogenetic Protein (BMP).

上颌重建需要两个生物材料的概念：一是用成骨材料填充骨缺损，二是保留骨缺损的空间，使成骨材料能够诱导成骨。本研究的目的是利用膨胀聚四氟乙烯（EPTFE）贴片^<TM>复合材料与骨形态发生蛋白（BMP）复合材料（EPTFE-BMP）膜作为骨膜替代物，研究实验骨腔的弹性以及生物材料与宿主骨之间的界面。在最初的计划中，人类BMP应用于这种复合材料，然而，它的聚合尚未取得进展。以牛骨基质中提取的1 mg BMP代替人骨BMP进行初步实验研究，将BMP与EPTFE补片表面结合。取日本白兔股骨周围骨膜，钻取直径5mm的孔，覆盖EPTFE-BMP复合膜。另一侧也单独使用EPTFE作为对照。植入后第2周和第4周处死动物，对标本进行组织学观察。结果表明，EPTFE-BMP复合膜组骨缺损被骨髓混合的新生骨组织填充，膜内微孔被新生骨填充。EPTFE单独组也观察到相同的新骨形成。结果表明，EPTFE- bmp复合膜组与单独使用EPTFE组无明显差异。

Two concepts of biomaterials are necessory for the maxillary reconstruction : one is to fill the bone defect by osteogenetic materials, and second is to keep the bone defect space for which the former material would be able to induce the osteoinductivity.The aim of this study is to investigate the os Resity of the experimental bone cavity and the interface between biomaterials and host bone using Expanded polytetrafloroethylene (EPTFE) Patch^<TM> composite with Bone morphogenetic protein (BMP) composite (EPTFE-BMP) membrane as a periosteum substitute. At the initial planning, fuman BMP was applied with this composite material, however, polification of it has noe been advanced.One mg of BMP extracted from bovine bone matrix was used for preliminary experimental study instead of fuman BMP.BMP was applied with the surface of the EPTFE Patches. Drill hole of 5mm in diameter was made with removal of surrounding periosteum in the femur of Japanese white rabbit and covered with EPTFE-BMP composite membrane. EPTFE alone was also used as a control on the other side. Two week and four week after implantation, all animals were sacrificed and the specimens were observed histologically. The results demonstrated that bone defect of the EPTFE-BMP composite membrane group was filled with new bone tissue mixed with bone marrow, and micro pores of the inner portion of the membrane were filled with new bone. Same new bone formation was also observed on the EPTFE alone group. The result demonstrated that there was no difference between EPTFE-BMP composite membrane and EPTFE alone group.