Chemical Study on the Interaction of Polyphenols with Co-existing Proteins

多酚与共存蛋白质相互作用的化学研究

• 批准号: 06672099
• 负责人: ITO Hideyuki
• 金额: $ 1.28万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672099/
• 关键词: Polyphenol; Protein; Interaction; Tannin; Conformation; HIV reverse transcriptase; Lipase; Enzyme inhibition; コンフォーメーション; xanthine oxidase; 速度論

We recently isolated a large number of tannins from various medicinal plants, and found their various biological activities including anti-virus, host-mediated antitumor activity, and inhibitory effects on several enzymes. Although these activities have often been regarded as the results of unspecific binding activity of polyphenolic compounds with proteins, the strengths of these activities have been found to differ depending on the structure of each polyphenol. In order to elucidate the interaction of polyphenols with co-existing proteins which should be a basis of their activities, we examined the kinetic aspects in the inhibitory effect of polyphenols on xantine oxidase, HIV reverse transcriptase and lipase. These results indicated that polyphenols exhibit the inhibition in a competitive, non-competitive or uncompetitive manner according to the difference of the chemical structure. In addition, the conformational analysis of polyphenols having an open-chain glucose and gluconic acid core in the molecule, which were newly isolated from several plants, gave the interesting findings on the interaction between polyphenols and proteins in the solution.

我们最近从各种药用植物中分离出大量单宁，并发现它们具有多种生物活性，包括抗病毒、宿主介导的抗肿瘤活性以及对多种酶的抑制作用。尽管这些活性通常被认为是多酚化合物与蛋白质非特异性结合活性的结果，但已发现这些活性的强度根据每种多酚的结构而不同。为了阐明多酚与共存蛋白质的相互作用（这应该是其活性的基础），我们检查了多酚对黄嘌呤氧化酶、HIV逆转录酶和脂肪酶的抑制作用的动力学方面。这些结果表明，多酚根据化学结构的差异以竞争性、非竞争性或非竞争性方式表现出抑制作用。此外，对从几种植物中新分离出的分子中具有开链葡萄糖和葡萄糖酸核心的多酚进行构象分析，得出了多酚与溶液中蛋白质之间相互作用的有趣发现。

项目成果

Takashi YOSHIDA: "A New Dimeric Hydrolyzable Tannin from Camellia oleifera." Phytochemistry. 37. 241-244 (1994)

Takashi YOSHIDA：“来自油茶的新型二聚水解单宁。”

Tsutomu HATANO,LiHAN,Shoko TANIGUCHI,Takuo OKUDA,Yoshinobu KISO,Takaharu TANAKA,and Takashi YOSHIDA: "Camelliatannin D,A New Inhibitor of Bone Resorption, from Camellia japonica." Chem.Pharm.Bull.43(11). 2033-2035 (1995)

Tsutomu HATANO、LiHAN、Shoko TANIGUCHI、Takuo OKUDA、Yoshinobu KISO、Takaharu TANAKA 和 Takashi YOSHIDA：“Camelliatannin D，一种新的骨吸收抑制剂，来自山茶花。”

毛利和子: "タンニンと共存物質との相互作用の効果(第8報)" 薬学雑誌. 115. 221-233 (1995)

Kazuko Mouri：“单宁与共存物质之间相互作用的影响（第 8 次报告）”《制药杂志》115. 221-233 (1995)。

Hideyuki ITO: "New Pisicidal Triterpenes from Iris germanica," Chem. Pharm. Bull.43. 1260-1262 (1995)

Hideyuki ITO：“来自德国鸢尾的新杀鱼三萜”，Chem。

Tsutomu HATANO: "Camellia tannin D, A New Inhibitor of Bone Resorption, from Camellia japonica." Chem. Phrarm. Bull.43. 2033-2035 (1995)

Tsutomu HATANO：“山茶单宁 D，一种新的骨吸收抑制剂，来自山茶花。”