INVESTIGATION OF OVERINTAKE AND DRUG INTERACTION OF FUNCTIONAL FOOD COMPONENTS

功能性食品成分的过量摄入和药物相互作用的调查

• 批准号: 17604005
• 负责人: ITO Hideyuki
• 金额: $ 2.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17604005/
• 关键词: FUNCTIONAL FOOD; DRUG FOOD INTERACTION; CRANBERRY; CYTOCHROME P-450; URINARY TRACT DESEASE; SAFETY OF FOOD STYLE; 薬物代謝酵素阻害

Functional foods have been used as prevention of several diseases in worldwide. Although its health benefit is noteworthy, the risk assessment of functional foods cannot be disregarded. In this study, I investigated the basic bioavailability of dietary polyphenol and the interaction between food components and medicine.Cranberry, which is rich in polyphenols including anthocyanins and proanthocyanidins, has been demonstrated to have various effects beneficial to human health including prevention of urinary tract infection. The bioavailability of cranberry polyphenols is little known. Forty-four metabolites in human urine after ingestion of cranberry juice were identified by using LC-ESI-MS/MS. Among metabolites, O-methylated proanthocyanidin A-2 were characterized as a new metabolite.Hydrolyzable tannins including ellagitannins occur in foods such as berries and nuts. Various biological activities, including antioxidant, antiviral and antitumor activities, have been noted and reported … More for ellagitannins, but the absorption and metabolism of purified ellagitannins are poorly understood. I investigated the characterization of urinary and intestinal microbial metabolites in rats after the ingestion of ellagitannin, geraniin. Rat urine sample was separated by column chromatography and/or preparative HPLC, to give seven metabolites, M1-M7. The structures of these metabolites were determined basis on spectroscopic data and chemical evidence. These compounds except for Ml were characterized as ellagitannin metabolites for the first time. Furthermore, among four major metabolites (M1-M4) in urine, M2 showed an antioxidant activity comparable to intact geraniin and related polyphenols.Interactions between food components and drugs have recently come into focus of interest. The interactions often occur as a result of inhibiting or inducing of the metabolic enzymes, cytochrome P450 (CYP). It is well known that concomitant intake with grapefruit juice increases the concentration of calcium antagonists in human by suppression of CYP3A4 activation. Cranberry juice has been traditionally used for the prevention of urinary tract infection. However, potentiated effects of warfarin following consumption of cranberry juice have been reported. The reasons for the interaction are mainly unknown. I study the inhibitory effects of cranberry extracts and ingredients, and related polyphenols on CYP2C9 and CYP3A4. Consequently, the gut microbial metabolites such as phenolic acids showed no effect. A part of compounds of coumarins, fiavones and flavonols exhibited a strong inhibitory activity.These results suggested that a part of dietary polyphenols might have the potential to inhibit the metabolism of drugs, so that further study should be investigated to clarify the clinical relevance. Less

在世界范围内，功能性食品已被用来预防多种疾病。虽然其对健康的益处值得注意，但功能性食品的风险评估不容忽视。在本研究中，我调查了膳食多酚的基本生物利用度以及食物成分与药物的相互作用。蔓越莓富含花青素和原花青素等多酚类物质，已被证明具有预防尿路感染等多种有益于人类健康的作用。小红莓多酚的生物利用度鲜为人知。采用LC-ESI-MS/MS联用技术对人体摄入酸莓汁后尿液中的44种代谢物进行了鉴定，其中O-甲基化原花青素A-2是一种新的代谢物。各种生物活性，包括抗氧化、抗病毒和抗肿瘤活性，已经被注意和报道…更多的是关于海葵单宁，但对纯化的海葵单宁的吸收和代谢知之甚少。我研究了大鼠摄入海葵单宁、天竺葵素后尿液和肠道微生物代谢产物的特征。大鼠尿样经柱层析和/或制备高效液相色谱分离，得到7种代谢物，M1-M7。根据波谱数据和化学证据确定了这些代谢物的结构。这些化合物除ML外，均为首次鉴定为鞣质的代谢物。此外，在尿液中的四种主要代谢物(M1-M4)中，M2显示出与完整的天竺葵素和相关多酚相当的抗氧化活性。食物成分与药物之间的相互作用最近成为人们关注的焦点。这种相互作用通常是由于代谢酶细胞色素P450(CYP)的抑制或诱导而发生的。众所周知，与西柚汁同时摄入会抑制细胞色素P3A4的激活，从而增加人体内钙拮抗剂的浓度。蔓越莓汁传统上被用于预防尿路感染。然而，有报道称，在饮用小红莓汁后，华法林的效果会增强。相互作用的原因主要不明。研究了小红莓提取物和成分以及相关多酚类物质对细胞色素P4502C9和细胞色素P3A4的抑制作用。因此，肠道微生物代谢产物，如酚酸，没有任何影响。部分香豆素、黄酮和黄酮醇类化合物表现出较强的抑制活性，提示部分膳食多酚可能具有抑制药物代谢的作用，其临床意义有待进一步研究。较少

项目成果

Tsutomu Hatano Neurotrophic effects of polyphenols and their metabolites

Tsutomu Hatano 多酚及其代谢物的神经营养作用

• 发表时间: 2008
• 作者: Hidevuki;Ito;Shu;X.-L
• 通讯作者: X.-L

Antioxidant Proanthocyanidin in Prune

西梅中的抗氧化原花青素

• 发表时间: 2007
• 作者: Yuka Kimura;Hideyuki Ito;Miyuki Kawaji;Takao Ikami;and Tsutomu Hatano
• 通讯作者: and Tsutomu Hatano

Antifungal Constituents from Santalum album of Indian Origin

来自印度原产檀香专辑的抗真菌成分

• 发表时间: 2006
• 作者: Hideyuki Ito;Tae Hoon Kim;Tsutomu Hatano;Keinosuke Okamoto;Michiko Arita;Hiroshi Kanzaki;Takahisa Machiguchi;and Takashi Yoshida
• 通讯作者: and Takashi Yoshida

ポリフェノールの化学構造と機能

多酚的化学结构和功能

• 发表时间: 2006
• 作者: 福田寿之;伊東秀之;波多野 力;吉田隆志;伊東秀之
• 通讯作者: 伊東秀之

Antibacterial activity of the constituents of Geranium thunbergil against Bacillus cereus

天竺葵成分对蜡样芽孢杆菌的抗菌活性

• 发表时间: 2005
• 作者: Yoshihiro;Yamada;Hidevuki;Ito;Tsutomu;Hatano;Keinosuke;Okamoto;Takashi;Yoshida
• 通讯作者: Yoshida