The Diversity of Retinylidene Proteins in Microorganisma

微生物中视黄基蛋白的多样性

• 批准号: 06672139
• 负责人: TAKAHASHI Tetsuo
• 金额: $ 1.41万
• 依托单位: Japan Advanced Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672139/
• 关键词: Phototaxis; Rhodopsin; Retinal; Chlamydomonas; Halobacteria; Archaebacteria; Behavior; Chemotaxis; Ectothiorhodospra halophila; Halobacterium halobium; Halobacterium salinarium; ISH; sensory vhodopsin; phoborhodopsin; H.halobium; phototaxis

The absorption spectrum of phoborhodopsin (PR) of Halobacterium salinarium (H.salinarium) exhibits unique features : (1) The absorption maximum is nearly 100-nm blue shifted from those of other bacterial rhodopsins. (2) It shows vibrational fine structure at ambient temperatures. Our recent results are : .1.13-demethyl retinal analog reconstitutes inverted photophobic responses in PR,while this analog confers no photochemical (of course no photobehavioral) activity to SR-I.2. Conformation at C6-C7 bond of the chromophore has been confirmed to be s-trans in PR,whereas the chromophore binding site in PR is not so restricted like other archaebacterial rhodopsins. This has been the conclusion of an analog-reconstitution study using the both trans and cis retinal analogs in which C6-C7 conformation was locked with either a 5-membered or a 7-membered ring. Interestingly, PR analog pigment has been reconstituted from 6-s-cis locked (with 7-membered ring) retinal, which has a C5=C6-C7=C8 structure close to the native retinal at the chromophore binding site of visual rhodopsin. However, this PR analog showed no vibrational fine structure.3. The primary sequence of PR in H.salinarium has been obtained quite recently, showing close homology to the related pigments of Natronobacterium pharaonis and Haloarcula vallismortis as well as bacteriorhodopsin (see also Zhang et al.Proc.Natl.Acad.Sci.93,8230-8235,1996) This and the above results confirmed that PR is a genuine member of the family of archaebacterial rhodopsins, leaving the unsolved problem of possible phylogenic relationship between archaebacterial rhodopsins and animal visual pigments.

盐酸盐盐（H.盐碱）的凤凰丁替蛋白（PR）的吸收光谱具有独特的特征：（1）吸收最大值近100 nm的蓝色转移来自其他细菌式杜宾蛋白的吸收。 （2）在环境温度下显示振动精细结构。我们最近的结果是：.1.1.13-二甲基视网膜模拟重构PR中倒出的辐射反应，而该模拟词没有光化学（当然没有光毛虫活性）与SR-i.2相关。发色团的C6-C7键的构象已证实是PR中的S-Trans，而PR中的发色团结合位点不像其他古细菌视紫红蛋白那样受到限制。这是使用反式和顺式视网膜类似物的模拟缔结研究的结论，其中C6-C7构象被5元或7元环锁定。有趣的是，PR模拟色素已从6-S-CIS锁定（带有7元环）视网膜中重构，该视网膜具有C5 = C6-C7 = C8 = C8结构，接近视觉Rhodopsin的发色团结合位点的天然视网膜。但是，该PR模拟没有显示振动的精细结构。3。 The primary sequence of PR in H.salinarium has been obtained quite recently, showing close homology to the related pigments of Natronobacterium pharaonis and Haloarcula vallismortis as well as bacteriorhodopsin (see also Zhang et al.Proc.Natl.Acad.Sci.93,8230-8235,1996) This and the above results confirmed that PR is a genuine member of the古细菌视紫红蛋白的家族，留下了尚未解决的问题，即考古视紫红蛋白和动物视觉色素之间可能存在系统的关系。

项目成果

Erata, M., Kubota, M., Takahashi, T., Inoue, I.and Watanabe, M.: "Ultrastructure and phototactic action spectra of two genera of cryptophyte flagellate algae, Cryptomonas and Chroomonas" Protoplasma. 188. 258-266 (1995)

Erata, M.、Kubota, M.、Takahashi, T.、Inoue, I. 和 Watanabe, M.：“隐藻鞭毛藻两个属的超微结构和趋光作用光谱，隐单胞菌属和色单胞菌属”原生质体。

Mayumi Erata: "Ultrastracture and Phototactic Action Spectra of Two Genera of Cryptophyte Flagellate Algae,Cryptomonas and Chroomonas" Protoplasma.

Mayumi Erata：“隐藻鞭毛藻、隐单胞菌和色单胞菌两个属的超结构和趋光作用光谱”原生质体。

Erata,M.,Kubota,M.,Takahashi,T.,Inoue,I,Watanabe,M.: "Ultrastracture and phototactic action spectra of two genera of cryptophyte flagellate algre,Cryptomonas and Chroomonas." Protoplasma. 188. 258-266 (1995)

Erata，M.，Kubota，M.，Takahashi，T.，Inoue，I，Watanabe，M.：“隐藻鞭毛虫两个属，隐单胞菌属和色单胞菌属的超结构和趋光作用光谱。”

Mayumi Evata: "Ultrastructure and phototartic action spectra of two genera of cryptophyto flagelate algae,Cryptomonas and Chroomonas" Protoplasma. 188. 258-266 (1995)

Mayumi Evata：“隐藻鞭毛藻两个属的超微结构和光致反应光谱，隐单胞菌和色单胞菌”原生质体。