Physiological and Biochemical Assessment of Membrane Barrier Function in Inflammatory Disease

评估炎症性肠病患者的膜屏障功能和电解质转运能力。用三硝基苯磺酸或乙酸制备炎症大鼠模型，比较电生理参数、膜电位差、短路电流（Isc）或膜电阻（Rm）与形态学炎症程度（损伤评分）的关系。各参数随得分的增加而降低，但相关性不显著，认为电生理分析更加客观准确。醋酸模型的Rm降低在制备后4天恢复到对照组，fitc -葡聚糖（FD-4）通透性增加在制备后7天恢复到对照组。因此，可以用上述方法在较短的时间内评估疾病的恢复情况。FD-4通透性的增加和Rm的降低提示细胞旁通路的扩大。采用上述方法评价双氯芬酸钠（DC）对细胞膜的损伤。大鼠灌注DC后发现细胞蛋白有释放。然而，由于茶碱的存在，Isc增加，表明膜代谢能力保持正常。综上所述，上述方法的应用可用于各种炎症性疾病的定量评估。

Membrane barrier function and transport ability for electrolytes were assessed in inflammatory bowel disease. For the inflammatory rat models prepared with trinirobenzene sulfonic acid or acetic acid, relation between the electro-physiological parameter, membrane potential difference, short circuit current (Isc), or membrane resistance (Rm), and the degree of morphological inflammation (damage score) was compared. The parameters decreased with increase in the scores, but the correlation was not significant, resulting that the electro-physiological analysis was considered more objective and accurate. For the acetic acid-models, the decreased Rm recovered to the control value in 4 days after the preparation and the increased permeability of FITC-dextran (FD-4) recovered to the control in 7 days. Accordingly, it was shown that recovery from the disease in the relatively short term can be assessed by the above method. The increase in the permeability of FD-4 and the decrease in Rm suggested the enlargement of the paracellular pathway. The membrane damage by diclofenac sodium (DC) was assessed by the above method. Release of cellular protein was found after perfusion of DC in the rat. However, Isc was increased by the presence of theophylline, suggesting that the membrane metabolizing ability remained normal. In conclusion, the application of the above method is useful in the quantitative assessment of various inflammatory disease.