Evaluation of water lead pollution in areas of fresh water using 5-aminolevulinic acid dehydratase (ALA-D) in the blood of carp Cyprinus carpio as an indicator of water lead pollution

以鲤鱼血液中的5-氨基乙酰丙酸脱水酶(ALA-D)作为水铅污染指标评价淡水地区水铅污染

• 批准号: 06680510
• 负责人: NAKAGAWA Hisaki
• 金额: $ 1.34万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680510/
• 关键词: 5-Aminolevulinic acid dehydratase; Carp blood; Crucian carp blood; Water lead pollution; Mercuric chloride; Lead chronic toxicity; Blood preservation method; Field survey; 5-アミノレブリン酸脱水酵素; 指標酵素; 環境規準値; 血液保存法; 活性標準化

Carp blood ALA-D was certified for use as an indicator of water lead pollution. In order to use ALA-D extensively for assessing water lead pollution, it is necessary to find a method which does not require HgCl_2 for the determination of ALA-D,and storage conditions for the blood which permit stability of and a standarized expression unit of ALA-D activity.The results are summarized as follows :1) In the case of a modified method which did not use HgCl_2, the reaction mixture was mixed and then left to stand for 10 min following the addition of 7% TCA solution. This was then repeated twice.2) Carp blood ALA-D was stable for at least 4 days when the blood was stored at 4ﾟC,in an ice bath or in a dry-ice bath. The three expression units of ALA-D activity, that is, per erythrocyte (RBC) , blood, and hemoglobin (Hb) , were related as follows :nmol PBG/ml RBC/h=3.6*nmol PBG/ml blood/h=0.256*nmol PBG/g Hb/hFish blood ALA-D has been usuful as an effective short-term indicator for evaluating t … More he chronic toxicity of water lead on fish. Conseqently, the validity of the environmental standard value of water lead in Japan (10 ppb) was evaluate from a 20-d exposure test.The results are summarized as follows :3) Judging from the inhibitory degree of the ALA-D,the lead standard value is a concentration that may cause chronic toxicity to carp.When we tried to survey water lead pollution in the fresh waters of northern Kyushu using carp, the carp were difficult to collect, however, crucian carp (Carassius auratus langsdorfii) could be collected easily. Accoringly a 3-wk exposure test of crucian carp to water lead was undertaken in order to evaluate the suitability of fish blood ALA-D as an indicator of water lead pollution.The results are summarized as follows :4) Blood ALA-D activity reflects the degree of lead contamination that crucian carp have suffered, and decreased to about 30% of that of control fish when the fish were exposed for 3 wks to water lead at a concentration of 10 ppb. Crucian carp blood ALA-D is useful as a indicator of water lead pollution.At present we are planning to assess the actual conditions of water lead pollution in areas of fresh water in northern Kyushu using crucian carp blood ALA-D. Less

鲤鱼血ALA-D已获得认证，可用作水铅污染的指标。为了使ALA-D广泛应用于水铅污染的评价，需要找到一种不需要HgCl_2的ALA-D测定方法，以及血液的保存条件，使ALA-D活性稳定和标准化表达单位，结果如下：1）在不使用HgCl_2的改良方法中，反应混合物混合后，加入7%TCA溶液静置10分钟。2）鲤鱼血ALA-D在4 ℃、冰浴或干冰浴中储存时至少稳定4天。ALA-D活性的三个表达单位，即每红细胞（RBC）、全血和血红蛋白（Hb），其关系如下：nmol PBG/ml RBC/h=3.6*nmol PBG/ml blood/h=0.256*nmol PBG/g Hb/h鱼血ALA-D可作为一种有效的短期指标，用于评价鱼血中ALA-D的活性。 ...更多信息 水铅对鱼类的慢性毒性。结果表明：（3）从ALA-D的抑制程度来看，日本水铅环境标准值（10 ppb）是对鲤鱼产生慢性毒性的浓度，在用鲤鱼调查北方淡水沃茨中，鲤鱼很难采集，而鲫鱼（Carassius auratus langsdorfii）很容易采集。结果表明：（4）鲫鱼血液中ALA-D活性反映了铅的污染程度，当铅浓度为10 ppb时，鲫鱼血液中ALA-D活性下降到对照组的30%左右，表明鲫鱼血液中ALA-D活性与铅浓度呈负相关。鲫鱼血ALA-D可作为水铅污染的指示剂，目前我们计划使用鲫鱼血ALA-D评估九州北方淡水地区水铅污染的实际情况。少

项目成果

Hisaki Nakagawa, Tsutomu Sato, Hirokatsu Kubo: "Method not requiring mercuric chloride for the determination of activity of 5-aminolevulinic acid dehydratase in blood of carp Cyprinus carpio" Fisheries Science. Vol.61. 97-99 (1995)

Hisaki Nakakawa、Tsutomu Sato、Hirokatsu Kubo：“不需要氯化汞即可测定鲤鱼血液中 5-氨基乙酰丙酸脱水酶活性的方法”渔业科学。

Hisaki Nakagawa, Tsutomu Sato, Hirokatsu Kubo: "Evaluation of chronic toxicity of water lead for carp Cyprinus carpio using its blood 5-aminolevulinic acid dehydratase" Fisheries Science. Vol.61. 956-959 (1995)

Hisaki Nakakawa、Tsutomu Sato、Hirokatsu Kubo：“利用鲤鱼血液 5-氨基乙酰丙酸脱水酶评估水铅对鲤鱼的慢性毒性”渔业科学。

中川久機・佐藤努・久保洋勝: "Evaluation of chronic tcoxicity of weater lead for carp Cyprinus carpio using its blood 5-aminoleulinic acid dehydratase" Fisheries Science. 61. 956-959 (1995)

Hisaki Nakakawa、Tsutomu Sato 和 Hirokatsu Kubo：“利用鲤鱼血液 5-氨基亮氨酸脱水酶评估毛衣铅对鲤鱼的慢性毒性”，渔业科学 61. 956-959 (1995)。

中川久機・佐藤努・久保洋勝: "Method not requiring mercuric chloride for the determination of activity of 5-amino-levulinic acid dehydratase in blood of carp cyprinus carpio" Fisheries Science. 61. 97-99 (1995)

Hisaki Nakakawa、Tsutomu Sato 和 Hirokatsu Kubo：“不需要氯化汞即可测定鲤鱼血液中 5-氨基乙酰丙酸脱水酶活性的方法”渔业科学 61. 97-99 (1995)。

中川久機,佐藤努,久保洋勝: "Evaluation of chronic toxicity of water lead for carp Cyprinus carpio using its blood 5-aminalevulinic acid dehydratase" Fisheries Science. 61. 956-959 (1995)

Hisaki Nakakawa、Tsutomu Sato、Hirokatsu Kubo：“利用鲤鱼血液 5-氨基乙酰丙酸脱水酶评估水铅对鲤鱼的慢性毒性”，渔业科学 61. 956-959 (1995)。