Monitoring of genotoxicity of river water im high sensitive umu test system

高灵敏umu测试系统监测河水遗传毒性

• 批准号: 06680523
• 负责人: OHE Takeshi
• 金额: $ 0.77万
• 依托单位: Kyoto Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680523/
• 关键词: an O-acetyltransferase-overproducing strain; umu test; Ames test; Genotoxicity monitoring; blue rayon hanging method; River water; 遺伝毒性; 芳香族アミン; XAD-2樹脂カラム法; ニトロアレーン; XAD-2樹脂カラム吸着法

Genotoxicity of organic chemicals in river water was investigated using a highly sensitive umu test with an O-acetyltranferase-overproducing strain Salmonella typhimurium NM2009. Organic chemicals in river water was sampled by blue rayon hanging method and XAD-2 resin column methods. Locations downstream from wastewater treatment plants showed high SOS-inducing activity in both the absence and the presence of a metabolic activation system, suggesting the occurrence of direct-acting nitroarenes and indirect-acting aminoarenes. The present results demonstrate that a high sensitive umu test using an O-acetyltransferase-overproducing strain is a useful bioassay system for the detection of trace amounts of nitroarenes and aminoarenes in the water environment.

采用高灵敏度umu试验对过量生产o -乙酰转移酶的鼠伤寒沙门菌NM2009进行了有机化学物质的遗传毒性研究。采用蓝色人造丝悬挂法和XAD-2树脂柱法对河水中的有机化学物质进行了取样。污水处理厂下游的位置在缺乏和存在代谢激活系统的情况下都显示出高的sos诱导活性，这表明存在直接作用的硝基芳烃和间接作用的氨基芳烃。本研究结果表明，使用过量生产o -乙酰转移酶的菌株进行高灵敏度umu试验是检测水环境中微量硝基芳烃和氨基芳烃的有效生物检测系统。

项目成果

Ohe Takeshi: "Evaluation of SOS-inducing activity with an-O-acetyltransteraseoverexpressing strain Selmonella,typlimuriun NM2009 for municipel river water and the identification of 1-nitropyrene" Water Science and Technology. 33(予定). (1996)

Ohe Takeshi：“用 an-O-乙酰基转移酶过度表达菌株 Selmonella，tylimuriun NM2009 对市政河水的 SOS 诱导活性进行评估以及 1-硝基芘的鉴定”水科学与技术 33（计划）。

大江武: "O-アセチル転移酵素高産生株を用いた高感度umuテストによる河川水の遺伝毒性モニタリング" 環境変異原研究. 17. 237-249 (1996)

Takeshi Oe：“使用 O-乙酰转移酶高产菌株进行高灵敏度 UMU 测试对河水的遗传毒性监测”环境诱变剂研究 17. 237-249 (1996)。

Takeshi Ohe: "Evaluation of SOS-inducing activity with an O-acetyl-transferase-cverexprssing strain Salmonella" Wat.Sci.Tech.33. 313-320 (1996)

Takeshi Ohe：“用 O-乙酰基转移酶表达菌株沙门氏菌评估 SOS 诱导活性”Wat.Sci.Tech.33。

Takeshi Ohe: "Evaluation of SOS-inducing activity with an O-acetyl-transferase-cverexprssing strain Salmonella" Wat.Sci.Tech.33. 313-320 (1996)"typhimurian NM2009 for municipal river water and the identification of 1-nitropyrene"

Takeshi Ohe：“用 O-乙酰基转移酶表达菌株沙门氏菌评估 SOS 诱导活性”Wat.Sci.Tech.33。

Takeshi Ohe: "Monitoring of genotoxicity of river water in high sensitive umu test system using Salmonella typhimurium NM2009 having high O-acetyltransferaseactivity." Environ.Mut.Res.Commun.17. 237-249 (1996)

Takeshi Ohe：“使用具有高 O-乙酰转移酶活性的鼠伤寒沙门氏菌 NM2009 在高灵敏度 UMU 测试系统中监测河水的遗传毒性。”