Mutagenicity evaluation of river waters in the NorthAmerica and Japan

北美和日本河水致突变性评价

• 批准号: 11680562
• 负责人: OHE Takeshi
• 金额: $ 1.34万
• 依托单位: Kyoto Women's Junior College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680562/
• 关键词: River water; Blue rayon hanging method; Mutagenicity; Salmonella typhimurium; YG1024 strain; Ames test; Amesテスト; 芳香族アミン高度受性株; ブルーキチンカラム; Sep pak CSPカラム; 北米の河川水; 芳香族アミン高感受性株; フレームシフト型変異原; 塩基対置換型変異原

The hanging technique using blue rayon, which specifically adsorbes mutagens with multicyclic planar structures, has advantages over the conventional method of bringing large volume of water back to the laboratoy for extraction. Therefore, the analysis of many samples from remote sites using the blue rayon hanging technique can be performed in a laboratory, although it has a disadvantage in terms of quantitative analysis. In this study, the blue rayon hanging technique was used to collect organic mutagens in river waters that flow through metropolitan areas in North America. Monitoring was performed at a total of 21 sites the Providence River (4 sites), the Charels River (2 sites), the Potomac River(6 sites), the St Lawrence River (5 sites), the Hudson River (3 sites), and the East River (1 site). Mutagenicity was evaluated by the Ames test using Salmonella strains TA98, TA100, YG1024, YG1041 and YG1042 with and without metabolic activation. The results demonstrated that strains YG1041 and YG1024 were much more sensitive than TA98 with S9 mix. Fourteen samples out of 21 were positive towards for YG1041 with S9 mix. Six samples among them gave 5,000 - 20,000 revertants per gram of blue rayon equivalent. YG1042 was also more sensitive than TA100. Eleven samples were positive in YG1042 with S9 mix. The highest activity was 10,300 revertants per gram of blue rayorfequivalent. The overall results showed that the water in rivers flowing through metropolitan areas contained some frameshift-type aromatic amine mutagens, although their mutagenicity was lower compared with river's data in Japan. Further studies were done to evaluate mutagenicity of river waters by blue-chitin column and Sep-pak CSP800 column. We conclude that both blue-chitin column and Sep-pak CSP800 column are efficient for quantitative assesment of mutagenicity of river waters and the blue rayon hanging method is the most convenient to assess mutagenic characteristics of river waters in remote areas.

使用蓝色人造丝的悬挂技术，专门吸附具有多环平面结构的诱变剂，与将大量水带回实验室进行提取的传统方法相比，具有优势。因此，使用蓝色人造丝悬挂技术对来自偏远地区的许多样品进行分析可以在实验室进行，尽管它在定量分析方面存在缺点。在这项研究中，蓝色人造丝悬挂技术被用于收集流经北美大都市地区的河水中的有机诱变剂。监测共在21个地点进行：普罗维登斯河（4个地点）、查尔斯河（2个地点）、波托马克河（6个地点）、圣劳伦斯河（5个地点）、哈德逊河（3个地点）和东河（1个地点）。以TA98、TA100、YG1024、YG1041和YG1042沙门氏菌为材料，在代谢激活和不代谢激活的情况下，采用Ames试验评价其致突变性。结果表明，菌株YG1041和YG1024与S9混合对TA98的敏感性明显高于TA98。21个样品中有14个样品对YG1041和S9混合物呈阳性。其中6个样品每克蓝色人造丝相当于含有5000 - 20000个回复剂。YG1042的敏感性也高于TA100。11份样品YG1042与S9混合阳性。最高的活性是每克蓝光或当量10300个反射物。总体结果表明，流经大都市地区的河流中含有一些移位型芳香胺诱变剂，但其诱变性低于日本的河流数据。采用蓝甲壳素色谱柱和Sep-pak CSP800色谱柱对河水进行诱变性评价。结果表明，蓝几丁质色谱柱和Sep-pak CSP800色谱柱均可用于河流水体致突变性的定量评价，蓝粘胶挂法是评价偏远地区河流水体致突变性的最简便方法。