DNA Probes That Exhibit Intense Fluorescence Upon Binding to DNA/RNA

与 DNA/RNA 结合后表现出强荧光的 DNA 探针

• 批准号: 06680564
• 负责人: YAMANA Kazushige
• 金额: $ 1.34万
• 依托单位: Himeji Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680564/
• 关键词: DNA porbe; oligonucleotide; fluorescent dye; dansyl; pyrene; オリゴヌクレオチド誘導体; ホスホロアミダイト体; DNA自動合成

The research efforts have been directed toward the development of new DNA probes that exhibit intense fluorescence upon binding to the targeted sequences in DNA/RNA.We designed two different oligonucleotide derivatives which are covalently linked to fluorescent dyes. One is the oligonucleotide carring a dansyl group at the sepcified sugar residue via a relatively short linker. This probe exhibits enhanced fluorescence and blue-shift in the emission when it binds to the complementary DNA.The other consists of two oligonucleotides possessing a pyrenylmethyl substituent at the 3' or 5' terminal hydroxyl function. A new method for introduction of the pyrene group into the positions has been established. The detail properties of these oligomers are now under investigation.We anticipate that the present DNA probes should provide a novel tool for detecting or discriminating DNA/RNA sequences in a homogeneous system, thereby affording an effective way to monitor the activity of gene by measuring expressed mRNA in several living cells.

研究的目的是开发新的DNA探针，使其与DNA/RNA中的目标序列结合时显示出强烈的荧光。我们设计了两种不同的寡核苷酸衍生物，它们与荧光染料共价连接。一种是通过相对较短的接头在发酵的糖残基上携带丹磺酰基的寡核苷酸。当它与互补DNA结合时，表现出增强的荧光和发射的蓝移。另一种是由两个在3‘或5’端羟基上具有吡喃甲基取代基的寡核苷酸组成的。建立了一种新的位置引入芘基团的方法。这些寡聚体的详细性质正在研究中。我们预计，目前的DNA探针将提供一种新的工具来检测或区分均一系统中的DNA/RNA序列，从而提供一种通过测量几个活细胞中表达的mRNA来监测基因活性的有效方法。

项目成果

Kazushige Yamana, Rie Aota, and Hidehiko Nakano: "Oligonucleotides Having Covalently Linked Anthracene at Specific Sugar Residue ; Differential Binding to DNA and RNA and Fluorescence Properties" Tetrahedron Letters. 36. 8427-8430 (1995)

Kazushige Yamana、Rie Aota 和 Hidehiko Nakano：“在特定糖残基处共价连接蒽的寡核苷酸；与 DNA 和 RNA 的差异结合以及荧光特性”四面体字母。

Masanori Kitamura et al.: "Chiral Phosphoromorpholidate Derivatives of Oligothymidyalte and Their Hybridization Abilities" Nucleosides and Nucleotides. 11. 473-482 (1992)

Masanori Kitamura 等人：“Oligothymidyalte 的手性磷酸吗啉衍生物及其杂交能力”核苷和核苷酸。

Kazushige Yamana et al.: "Synthesis of A New Photoisomerizable Linker for Connecting Two Oligonucleotide Segments" Tetrahedron Letters. 37. 637-640 (1996)

Kazushige Yamana 等人：“用于连接两个寡核苷酸片段的新型光致异构化连接子的合成”四面体字母。

山名一成: "糖部修飾オリゴヌクレオチドの合成" 蛋白質核酸酵素. 40. 1349-1356 (1995)

Kazunari Yamana：“糖修饰寡核苷酸的合成”蛋白质核酸酶。40。1349-1356（1995）

Kazushige Yamana,et al.: "A New Method for Introduction of A Pyrene Group into A Terminal Position of An Oligonucleotide" Tetrahedron Letters. 35. 2555-2558 (1994)

Kazushige Yamana 等人：“将芘基团引入寡核苷酸末端位置的新方法”四面体字母。