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Molecular Mechanism of Epithelial-Mesenchymal Interaction

上皮-间质相互作用的分子机制

基本信息

批准号：
06680716
负责人：
FUKAMACHI Hiroshi
金额：
$ 1.28万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680716/
关键词：
Epithelial-Mesenchymal Interaction Hepatocyte Growth Factor Keratinocyte Growth Factor RT-PCR Primary Culture Stomach Pepsinogen Cathepsin E 小腸 HGF 表皮成長因子 KGF 形態形成上皮細胞増殖因子基質分化

项目摘要

1.Glandular stomach epithelial cells expressed Cathepsin E but not pepsinogen when cultured in the absence of mesenchyme, but they expressed pepsinogen when cultured with mesenchyme. We thus concluded that the differentiation of glandular stomach epithelial cells is determined by the epithelial-mesenchymal interaction.2.Hepatocyte growth factor (HGF) region-specifically stimulated proliferation of gastro-intestinal epithelial cells in primary culture. RT-PCR analysis showed that gastro-intestinal mesenchymes and epithelia express HGF and HGF receptors, respectively. We thus concluded that HGF is involved in the epithelial-mesenchymal interaction in gastro-intestinal organs.3.Keratinocyte growth factor (KGF) also region-specifically stimulated proliferation of gastro-intestinal epithelial cells in primary culture. RT-PCR analysis showed that gastro-intestinal mesenchymes and epithelia express KGF and KGF receptors, respectively. We thus concluded that KGF is also involved in the epithelial-mesenchymal interaction in gastro-intestinal organs.4.The role of HGF and KGF in the development of glandular stomach was investigated by quantitative RT-PCR.Genes for HGF and HGF receptor were expressed at the morphogenetic period, and those for KGF and KGF receptor after the morphogenetic period. Thus we concluded that these two growth factors play different roles in developing glandular stomachs.

1.腺胃上皮细胞表达组织蛋白酶E，但不表达胃蛋白酶原培养时，在没有间充质，但他们表达胃蛋白酶原培养时，间充质。结论：腺胃上皮细胞的分化是由上皮-间质相互作用决定的。2.肝细胞生长因子（HGF）区域特异性刺激原代培养的胃肠上皮细胞增殖。RT-PCR分析表明，胃肠道间质和上皮细胞表达HGF和HGF受体。结论：HGF参与了胃肠道上皮细胞与间质细胞的相互作用。3.角质细胞生长因子（KGF）对原代培养的胃肠道上皮细胞具有区域特异性的促增殖作用。RT-PCR分析表明，胃肠道间充质和上皮细胞表达KGF和KGF受体。结论：KGF还参与了胃肠道上皮细胞与间质细胞的相互作用。4.通过定量RT-PCR研究了HGF和KGF在腺胃发育中的作用，发现HGF和HGF受体基因在腺胃形态发生期表达，KGF和KGF受体基因在形态发生期后表达。因此，我们得出结论，这两种生长因子在腺胃发育中发挥不同的作用。