Identification and characterization of human gastric cancer stem cells

人胃癌干细胞的鉴定和表征

• 批准号: 22501005
• 负责人: FUKAMACHI Hiroshi
• 金额: $ 2.41万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22501005/
• 关键词: がん細胞の特性; 幹細胞; 胃がん; がん幹細胞; 造腫瘍性; FACS; ヌードマウス; 無血清培養; sphere; 細胞表面抗原; Xenograft; spheroids; ヒト

We established xenograft lines where human gastric tumor tissues maintain their differentiation and tumorigenesis. Using these lines, we identified a marker which was specifically expressed by gastric cancer stem cells. We also confirmed that the marker was expressed by cancer stem cells which were obtained from newly-dissected gastric cancers. Moreover, we established serum-free culture system where gastric cancer stem cells proliferate to form spheres, structures specific to stem cells. These sphere-forming cells expressed the antigen and were highly tumorigenic. We are now examining the mechanism how their tumorigenicity is controlled.

我们建立了人胃肿瘤组织保持其分化和肿瘤发生的异种移植系。利用这些细胞系，我们鉴定了胃癌干细胞特异性表达的标志物。我们还证实了该标记物由从新解剖的胃癌中获得的癌症干细胞表达。此外，我们建立了无血清培养体系，胃癌干细胞增殖形成干细胞特异性结构的球体。这些球形成细胞表达抗原，并且具有高度致瘤性。我们现在正在研究它们的致瘤性是如何被控制的机制。

项目成果

生物学辞典

生物学词典

• 作者: 浜田盛久;川本竜彦;高橋栄一;藤井敏嗣;江崎洋一
• 通讯作者: 江崎洋一

Synergistic tumor suppressor activity of E-cadherin and p53 in a conditional mouse model for diffuse-type gastric cancer

E-钙粘蛋白和 p53 在弥漫型胃癌条件小鼠模型中的协同抑癌活性

• 发表时间: 2011
• 作者: Shimada;S.;Mimata;A.;Akiyama;Y.;Fukamachi;H.;Yuasa Y.
• 通讯作者: Yuasa Y.

miR-212 is downregulated and suppresses methyl-CpG-binding protein MeCP2 in human gastric cancer

• DOI: 10.1002/ijc.25126
• 发表时间: 2010-09-01
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Wada, Rie;Akiyama, Yoshimitsu;Yuasa, Yasuhito
• 通讯作者: Yuasa, Yasuhito

Runx3 protects gastric cepithelial cells against EMT and Lgr5-expressing tumorigenic subpopulation

Runx3 保护胃上皮细胞免受 EMT 和表达 Lgr5 的致瘤亚群的影响

• 发表时间: 2011
• 作者: Ito;Y.;Ito;K.;Fukamachi;H.
• 通讯作者: H.

Loss of E-cadherin in parietal cells affects gastric epithelial cell polarity, growth and differentiation, resulting in a pre-malignant condition in mice

壁细胞中 E-钙粘蛋白的缺失会影响胃上皮细胞的极性、生长和分化，导致小鼠出现癌前状态

• 发表时间: 2010
• 作者: Mimata;A.;Fukamachi;H.;Eishi;Y.;Yuasa;Y.
• 通讯作者: Y.