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Confocal laser microscopic study on the mechanism of the migration of dopamine neurons in the mouse mesencephalon.

共聚焦激光显微研究小鼠中脑多巴胺神经元迁移机制。

基本信息

批准号：
06680745
负责人：
KAWAMURA Koki
金额：
$ 1.54万
依托单位：
KEIO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680745/
关键词：
Substantia Nigra Dopamine Neuron Neuronal Migration DiI Tyrosine Hydroxylase Tenascin Radial glia Ll マウス発生

项目摘要

Dopamine neurons in the mouse embryonic mesencephalon has been shown to migrate ventrally and then laterally to form the anlage of the substantia nigra pars compacta. We have examined the migratory pattern of these neurons by using immunohistochemistry and demonstrated that the ventral migration of these neurons is guided by the radial glial fibers bearing a extracellular matrix molecule, tenascin, and lateral migration is along the nerve fibers expressing cell adhesion molecules, N-CAM-H and L1 (Kawano, Ohyama, Kawamura and Nagatsu, Dev. Brain Res. 86 : 101-103,1995) .Employing the confocal laser microscope and three dimensional computer analyzer, we have observed nerve fibers and cellular interactions between migrating dopamine neurons and guiding structure. (1) When fluorescent tracer, DiI,was injected into the ventromedial part of the paraformaldehyde-fixed E12 mesencephalon, the cells of origin of the guiding fibers were revealed tobe localized in the lateral part of the, mesencephalon (Kawano, Ohyama, Kawamura and Nagatsu, Dev. Brain Res. 86 : 101-103,1995) . (2) The sections of E12 mesencephalon were treated with double fluorescent labeling in which dopamine neurons were demonstrated with anti-tyrosine hydroxylase and guiding nerve fibers with DiI.When these preparations were observed using the confocal laser microscope, the cell bodies of immature dopaminergic neurons labeled with tyrosine hydroxylase migrated laterally in contact with DiI-labeled nerve fibers. These findings indicate that specific structures expressing specific molecules which appear in the developing brain play a key role in the neuronal migration.

小鼠胚胎中脑的多巴胺神经元先向腹侧迁移，然后向外侧迁移，形成黑质背侧部的原基。我们已经通过使用免疫组织化学检查了这些神经元的迁移模式，并且证明这些神经元的腹侧迁移由带有细胞外基质分子腱生蛋白的放射状胶质纤维引导，并且侧向迁移是沿着表达细胞粘附分子N-CAM-H和L1的神经纤维（Kawano，Ohyama，Kawamura和Nagatsu，Dev.第86章：你是谁101- 103，1995）。利用共聚焦激光显微镜和三维计算机分析仪，我们观察了迁移的多巴胺神经元和引导结构之间的神经纤维和细胞相互作用。(1)当将荧光示踪剂DiI注射到去甲肾上腺素固定的E12中脑的腹内侧部分时，显示引导纤维的起源细胞位于中脑的外侧部分（Kawano，Ohyama，Kawamura和Nagatsu，Dev. 86：101- 103，1995）。(2)E12中脑切片用双荧光标记法处理，其中多巴胺能神经元用抗酪氨酸羟化酶标记，引导神经纤维用DiI标记。当这些制备物用共聚焦激光显微镜观察时，未成熟的多巴胺能神经元用酪氨酸羟化酶标记的细胞体与DiI标记的神经纤维接触，向侧面迁移。这些发现表明，在发育中的大脑中出现的表达特定分子的特定结构在神经元迁移中起关键作用。