Reconstruction of neural tissue by means of transplantation of immortalized cell line generated by transfer of oncogene (tsA58)

将永生化细胞系V1作为未成熟神经细胞模型用于移植实验，并用组织学方法观察宿主-移植物在小鼠脑内的相互作用。该细胞系由逆转录病毒介导将温度敏感的癌基因tsA58导入小鼠胚胎间脑原代培养，在39ﾟ℃下表现为神经元或神经胶质细胞分化，与小鼠体温一致。然后，将该细胞系立体定向移植到小鼠脑的小脑和海马区，并分析了移植细胞的行为与移植部位和宿主脑的发育阶段有关。当移植到发育中的大脑中时，该细胞系迁移和Wa…在小脑和海马齿状回的浦肯野细胞层中，S分布较多，并与宿主皮质星形胶质细胞的排列密切相关。该细胞系移植到成年小脑后，在浦肯野细胞层也表现出迁移和排列。相比之下，移植到成年海马区的V1细胞没有表现出任何特殊的排列。本研究结果表明，移植的V1细胞可以整合到宿主大脑的发育过程中，并与皮质结构相对应的排列。发育中的宿主脑的神经胶质结构被认为与移植细胞的排列有关。相比之下，宿主-移植物的相互作用在成年小鼠的小脑和海马区之间显示出差异。上述发现表明，宿主大脑整合移植细胞的可塑性可能在成人大脑的受限区域保持。这项研究表明，通过移植未成熟的神经细胞来重建成人大脑的可能性。较少

An immortalized cell line V1 was applied to the transplantation experiments as the model of immature neural cells and the host-graft interaction in the mouse brain was examined by histological methods. This cell line was generated by retrovirus mediated transduction of temperature-sensitive oncogene tsA58 into the primary culture of mouse embryonic diencephalon and showed neuronal or glial differentiation at 39ﾟC which corresponds to the mouse body temperature.When this cell line was transplanted into the adult diencephalon, the site of origin of this cell line, they showed neuronal differentiation as revealed by the expression of neurofilament immunoreactivity. Then, this cell line was transplanted stereotaxically into the cerebellum and hippcampus of the mouse brain and the behavior of the transplanted cells was analyzed with regard to the site of transplantation and the developmental stage of the host brain. When transplanted into the developing brain, this cell line migrated and wa … More s stratified in Purkinje cell layr of the cerebellum and hippocampal dentate gyrus, and close association with the arrangement of the host cortical astroglia was found. When transplanted into the adult cerebellum, this cell line also showed migration and arrangement in Purkinje cell layr. In contrast, when transplanted in the adult hippocampus, this cell line did not show any particular arrangement.The results of the present study suggest that transplanted cell line V1 can be integrated into the developmental processes of the host brain and is arranged corresponding to the cortical structure. The glial framework of the developing host brain is considered to be involved in the arrangement of the transplanted cells. In contrast, host-graft interaction showed differences between the cerebellum and hippocampus of the adult mouse. Above findings suggest that the plasticity of the host brain to integrate the grafted cells might be maintained in the restricted area of the adult brain. The possibility to reconstruct the adult brain by the transplantation of immature neural cells was shown in this study. Less