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Reconstruction of neural tissue by means of transplantation of immortalized cell line generated by transfer of oncogene (tsA58)

通过移植癌基因（tsA58）产生的永生化细胞系来重建神经组织

基本信息

批准号：
04670873
负责人：
KAWAMURA Koki
金额：
$ 1.41万
依托单位：
Keio University, School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

An immortalized cell line V1 was applied to the transplantation experiments as the model of immature neural cells and the host-graft interaction in the mouse brain was examined by histological methods. This cell line was generated by retrovirus mediated transduction of temperature-sensitive oncogene tsA58 into the primary culture of mouse embryonic diencephalon and showed neuronal or glial differentiation at 39ﾟC which corresponds to the mouse body temperature.When this cell line was transplanted into the adult diencephalon, the site of origin of this cell line, they showed neuronal differentiation as revealed by the expression of neurofilament immunoreactivity. Then, this cell line was transplanted stereotaxically into the cerebellum and hippcampus of the mouse brain and the behavior of the transplanted cells was analyzed with regard to the site of transplantation and the developmental stage of the host brain. When transplanted into the developing brain, this cell line migrated and wa … More s stratified in Purkinje cell layr of the cerebellum and hippocampal dentate gyrus, and close association with the arrangement of the host cortical astroglia was found. When transplanted into the adult cerebellum, this cell line also showed migration and arrangement in Purkinje cell layr. In contrast, when transplanted in the adult hippocampus, this cell line did not show any particular arrangement.The results of the present study suggest that transplanted cell line V1 can be integrated into the developmental processes of the host brain and is arranged corresponding to the cortical structure. The glial framework of the developing host brain is considered to be involved in the arrangement of the transplanted cells. In contrast, host-graft interaction showed differences between the cerebellum and hippocampus of the adult mouse. Above findings suggest that the plasticity of the host brain to integrate the grafted cells might be maintained in the restricted area of the adult brain. The possibility to reconstruct the adult brain by the transplantation of immature neural cells was shown in this study. Less

将永生化细胞系V1作为未成熟神经细胞的模型应用于移植实验，并通过组织学方法检查小鼠脑中的宿主-移植物相互作用。该细胞系是通过逆转录病毒介导的温度敏感性癌基因tsA 58转导到小鼠胚胎间脑的原代培养物中而产生的，并且在39 ℃（对应于小鼠体温）下显示神经元或神经胶质分化。它们表现出神经丝免疫反应性表达所揭示的神经元分化。然后，将该细胞系立体定位地移植到小鼠脑的小脑和海马中，并就移植部位和宿主脑的发育阶段分析移植细胞的行为。当移植到发育中的大脑中时，这种细胞系迁移， ...更多信息在小脑和海马齿状回的浦肯野细胞层中有明显的分层现象，并与宿主皮层星形胶质细胞的排列密切相关，移植到成人小脑后，该细胞系在浦肯野细胞层中也有迁移和排列现象。与此相反，当移植到成年海马，该细胞系并没有表现出任何特殊的arrangement.The本研究的结果表明，移植的细胞系V1可以整合到宿主大脑的发育过程中，并安排相应的皮质结构。发育中的宿主脑的神经胶质框架被认为参与移植细胞的排列。相反，宿主-移植物相互作用显示成年小鼠小脑和海马之间的差异。上述结果提示，移植细胞整合宿主脑的可塑性可能在成人脑的限制区得以维持。本研究为未成熟神经细胞移植重建成人脑组织提供了可能性。少