Effects of lighting condition to the behavioral and drug sensitive rhythms in the rat

将雄性Wistar-Imamichi(W-I)和BN大鼠置于不同强度(10,100和10001UX)的光照下。光照周期为LD=12：12(6：00灯亮)。本研究的主要结果如下：(1)光照强度对W-I的步行活动有影响。然而，在BN大鼠中，这种影响相当小。观察到在10lux以下的W-I组在照明期有较高的活动能力。除上述现象外，两种系大鼠在不同光照强度下的日常活动行为节律模式均无差异。(2)W-I和BN于9：00和18：00分别腹腔注射戊巴比妥钠50 mg/kg，三组大鼠在不同光照强度下的麻醉效果无差异。但9：00 PB注射的麻醉效应持续时间长于18：00注射的麻醉效应(3)9：00皮下注射可卡因10 mg/kg，10勒克斯下的急性效应最强。(4)在W-I品系，18：00注射可卡因的急性效应持续时间长于9：00。在W-I模式下，10勒克司下的急性效应最强。在不同光照强度下，分别于9、12、18、21、24：00取血进行生化分析。总蛋白、葡萄糖、GTP等含量因光照强度不同而有一定差异。

Male Wistar-Imamichi (W-I) and BN rats were placed under the various intensity of illumination (10,100 and 1000 1ux). Lighting cycle was LD=12 : 12 (6 : 00 lights on). Present study leads the following results.(1) Ambulatory activity of W-I was affected by the intensity of illumination. However, this effect was rather small in BN rats. It was observed to higher ambulatory activity during lighting period in the group of W-I under the 10 lux. Except this phenomenon, there was no difference in the daily behavioral rhythm pattern of ambulatory activity under the various intensity of illumination in the both rat strain.(2) In pentobarbital sodium 50mg/kg i.p.injection to W-I and BN at 9 : 00 and 18 : 00, there was no difference in the anesthetic effect among the three groups under the various intensity of illumination. However, anesthetic effect lasted longer in the rats of 9 : 00 PB injiection than that of 18 : 00 injection(3) In cocaine 10mg/kg s.c.injection to the both strain at 9 : 00, the strongest acute effect was observed under the 10 lux.(4) In W-I strain, acute effect of cocaine injection at 18 : 00 continued longer than that at 9 : 00. In W-I,the strongest acute effect was observed under the 10 lux. However, acute effect was least under the 10 lux in BN.(5) Biochemical analysis was done with blood taken at 9,12,18,21 and 24 : 00 under the various intensity of illumination. There were some differences in the level of total protein, glucose, GTP et al due to the different intensity of illumination.