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Effects of lighting condition to the behavioral and drug sensitive rhythms in the rat

光照条件对大鼠行为和药物敏感节律的影响

基本信息

批准号：
06680825
负责人：
SHINODA Motoo
金额：
$ 0.45万
依托单位：
Gunma University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680825/
关键词：
lighting condition intensity of illumination spontaneous motor activity rats diurnal rhythm sensitivity pentobarbital cocaine 飲水行動

项目摘要

Male Wistar-Imamichi (W-I) and BN rats were placed under the various intensity of illumination (10,100 and 1000 1ux). Lighting cycle was LD=12 : 12 (6 : 00 lights on). Present study leads the following results.(1) Ambulatory activity of W-I was affected by the intensity of illumination. However, this effect was rather small in BN rats. It was observed to higher ambulatory activity during lighting period in the group of W-I under the 10 lux. Except this phenomenon, there was no difference in the daily behavioral rhythm pattern of ambulatory activity under the various intensity of illumination in the both rat strain.(2) In pentobarbital sodium 50mg/kg i.p.injection to W-I and BN at 9 : 00 and 18 : 00, there was no difference in the anesthetic effect among the three groups under the various intensity of illumination. However, anesthetic effect lasted longer in the rats of 9 : 00 PB injiection than that of 18 : 00 injection(3) In cocaine 10mg/kg s.c.injection to the both strain at 9 : 00, the strongest acute effect was observed under the 10 lux.(4) In W-I strain, acute effect of cocaine injection at 18 : 00 continued longer than that at 9 : 00. In W-I,the strongest acute effect was observed under the 10 lux. However, acute effect was least under the 10 lux in BN.(5) Biochemical analysis was done with blood taken at 9,12,18,21 and 24 : 00 under the various intensity of illumination. There were some differences in the level of total protein, glucose, GTP et al due to the different intensity of illumination.

将雄性Wistar-Imamichi（W-I）和BN大鼠置于不同强度的光照（10、100和1000 μ x）下。光照周期为LD=12：12（6：00开灯）。本研究得出以下结论。(1)步行活动受光照强度的影响。然而，这种影响是相当小的BN大鼠。在10 lux光照下，W-I组的步行活动明显高于对照组。除此现象外，两种品系大鼠在不同光照强度下的步行活动的日行为节律模式没有差异。(2)戊巴比妥钠50 mg/kg于9：00和18：00腹腔注射于W-I和BN，在不同光照强度下，三组麻醉效果无差异。（3）9：00时皮下注射可卡因10 mg/kg对两种品系大鼠的麻醉作用均以10 lux下的急性效应最强。(4)W-Ⅰ株18：00时注射可卡因的急性效应持续时间较9：00时长。在W-I中，在10 lux下观察到最强的急性效应。而BN在10 lux以下的急性效应最小。(5)分别于9、12、18、21、24：00在不同光照强度下取血进行生化分析。总蛋白、葡萄糖、GTP等含量因光照强度的不同而有所差异。