Establishment of cryo-preservation method for ovary and testis and its application to storage of wild mosue genes.

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1.Establishment of cryo-preservation method for ovary and its application to storage of wild mouse genesSuccessful freezing conditions of ovaries were detected in a series of experiments using laboratory mice. When the freezing method was applied to ovaries of MSM inbred strain derived from Japanese wild mice, mice derived from frozen ovaries were obtained, though litter sizes were very small.2.Establishment of cryo-preservation method for testis(1) Histological examinationFreezing injuries of newborn mouse testes were examined histologically at various points of a freezing course. A lot of germ cells retained a normal morphology in the program of slow cooling at a rate of 0.2 C/min till -40 C before immersion into LN_2.(2) Transplantation of frozen testisIn order to confirm the growth of germ cells and the production of spermatozoa, the frozen newborn testes were transplanted into adult testes. In the case of a freezing program described above, at 14 days after transplantation meiotic germ cells were observed and at six weeks spermatozoa were detected in the seminiferous tubules derived from the frozen testes.