Establishment of cryo-preservation method for ovary and testis and storage of wild mouse genes.

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This study aimed at an establishment of storage system for wild mouse genome by freezing of ovary and testis from a viewpoint of preservation of gene resources.1. Establishment of cryo-preservation method for ovary and strage of wild mouse genes.Successful freezing conditions of ovaries were detected in a series of experiments using laboratory mice. When the freesing method was applied to ovaries of MSM inbred strain derived from Japanese wild mice, mice derived from frozen ovaries were obtained, though litter sizes were very small.2. Establishment of cryo-preservation method for testisfreezing injuries of newborn mouse testes were examined histologically at various points of a freezing course. A lot of germ cells retained a normal morphology in the program of slow cooling til -40ﾟC before immersion into LN2.In order to confirm the growth of germ cells and the production of spermatozoa, the frozen newborn testis were transplanted into histocompatible adult testis. In the case of a freezing program described above, at 14 days after transplantation meiotic germ cells were observed and at six weeks spermatozoa were detected in the seminiferous tubules derived from the frozen testes, though it has not been confirmed whether they have a capacity of fertilization to oocytes.A reconstitution of thawed testis to a host reproductive system is under investigation.