Effect of in vivo treatment with anti-interleukin 2 receptor gamma chain antibody on antigen-induced eosinophilic brochial inflammation in mice.

抗白细胞介素2受体γ链抗体体内治疗对抗原诱导的小鼠嗜酸性支气管炎症的影响。

• 批准号: 06807054
• 负责人: IKEDA Hirotada
• 金额: $ 1.09万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06807054/
• 关键词: Gamma chain; Interleukin 2; Receptor; Eosinophil; Bronchial asthma; Interleukin 4; Mice; Antibody; 抗IL-2受容体γ鎖抗体; IL-2; IL-4; 卵白アルブミン感作マウス; 気管支肺胞洗浄液; IL-5

Using a model of ovalbumin-induced eosinophilic bronchial inflammation in sensitized mice, we examined the effect of in vivo blocking of the common gamma chain of interleukin 2 receptor by in vivo treatment with monoclonal antibody to this molecule. The antibody "TUGm2" has already been shown to have the inhibitory effect on cell growth induced by IL-4, IL-7 and IL-9 in vitro. The in vivo blocking of common gamma chain prevented antigen-induced eosinophil infiltration into the mouse trachea in terms of the tissue and bronchoalveolar lavage cell examinations. In contrast, pretreatment with this antibody at the dose of 1.4mg/body did not prevent antigen-induced accumulation of antigen-specific or non-specific IgE,IgG1 and IgG2a. Experiments with different doses of antibody are in progress. The RT-PCR studies of mRNA for IL-2 and IL-4 that may enable the elucidation of the possible change in TH1/TH2 cytokine pattern are also on going.

利用卵巢蛋白诱导的嗜酸性支气管炎症模型在敏化小鼠中，我们通过体内用单克隆抗体对该分子的单克隆抗体进行体内治疗，研究了白介素2受体的体内阻断的影响。抗体“ TUGM2”已经证明对IL-4，IL-7和IL-9体外诱导的细胞生长具有抑制作用。普通伽马链的体内阻断可阻止抗原诱导的嗜酸性粒细胞在组织和支气管肺泡灌洗细胞检查方面浸润到小鼠气管中。相比之下，该抗体在1.4mg/身体的剂量下进行预处理并不能阻止抗原诱导的抗原特异性或非特异性IgE，IgG1和IgG2A的积累。具有不同剂量抗体的实验正在进行中。 IL-2和IL-4的mRNA的RT-PCR研究可能会阐明Th1/Th2细胞因子模式可能变化的RT-PCR。