"Expression of c-fos protein in rat brain after occlusion of superior mesenteric artery (immunohistochemical investigation)"

“肠系膜上动脉闭塞后大鼠脑中 c-fos 蛋白的表达（免疫组织化学研究）”

• 批准号: 06807103
• 负责人: HASE Takanobu
• 金额: $ 1.22万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06807103/
• 关键词: c-fos; occlusion of the superior mesenteric artery; rat brain; immunohistochemistry; endotoxin; c‐fos; エンドトキシン; 抗利尿ホルモン

The expression of c-fos in brain and endotoxin level in plasma were studied at 0,2,4,6,8 hours after occlusion of the superior mesenteric atery of rat. In sham-operated rat brain, a few neuronal cells strained by immunohistochemistry for c-fos were scattered in the hypothalamic paraventricular, hypoyhalamic supraoptic and lateral habenular nuclei. In arterial occlusion treated rats, however, many c-fos positive neurons were observed in certain populations of various brain regions. These positive cells were first detected as early as 2 hours after the treatment. The regions possessing occlusion-induced c-fos positive neurons include the nucleus tractus solitalius, subfornical organ, locus coeruleus, dorsal motor nucleus of vagus, hypothalamic paraventricular, supraoptic and ventromedial nuclei, lateral habenular nucleus, and central nucleus of amygdala, In these animals, the plasma endotoxin were kept at low levels during early period but were rapidly increased 6 hours afer surgery. The results indicate that certain neuronal systems such as the limbic, hypothalamic, and brainstem regions appear to be involved in a pathophysiological response to the mesenteric arterial occlusion. The present neuroanatomical evidence may support the idea that the emotional change seen in human necrotizing enteritis and sepsis is due to a direct influence of sepsis-associated alterations on the central nervous system. Since, inaddition, such neuronal activation in brain preceedes the elevation of plasma endotoxin level, other unknown factors than endotoxin may conduct the influence to the brain.

观察大鼠上级肠系膜动脉阻断后0、2、4、6、8 h脑内c-fos表达及血浆内毒素水平的变化。假手术组大鼠脑组织中，在下丘脑室旁核、下丘视上核和外侧缰核内散在分布着少量c-fos阳性神经细胞。然而，在动脉闭塞治疗的大鼠，许多c-fos阳性神经元中观察到的各种脑区的某些群体。这些阳性细胞早在处理后2小时就被首次检测到。在孤束核、穹窿下器、蓝斑、迷走神经背侧运动核、下丘脑室旁核、视上核、腹内侧核、外侧缰核和杏仁中央核等部位有c-fos阳性神经元。结果表明，某些神经系统，如边缘系统，下丘脑，脑干区域似乎参与了肠系膜动脉闭塞的病理生理反应。目前的神经解剖学证据可能支持这样的观点：人类坏死性肠炎和脓毒症中观察到的情绪变化是由于脓毒症相关改变对中枢神经系统的直接影响。此外，由于脑内神经元的这种激活先于血浆内毒素水平的升高，因此，除内毒素外的其他未知因素也可能对脑产生影响。