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Modification of functional architecture of the visual cortex by a developmental pharmacological method

通过发育药理学方法改变视觉皮层的功能结构

基本信息

批准号：
06808087
负责人：
IMAMURA Kazuyuki
金额：
$ 1.22万
依托单位：
Osaka Bioscience Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06808087/
关键词：
subplate functional organization of the visual cortex cat ocular dominance column binocular competition ocular dominance plasticity the lateral geniculate nucleus development カイニン酸脳梁免疫組織化学逆行性標識線条皮質シナプス可塑性眼優位帯

项目摘要

Neuronal activity and function of subplate cells have been proposed to be involved in the formation of functional architecture of the visual cortex. We have studied the effects of kainic acid injected locally into the visual cortex of a few-days-old kitten. Electrophysiological and immunohistochemical examinations showed that the synaptic connections for normal visuocortical function was prevented to develop by this pharmacological manipulation. In order to examine in detail about the termination pattern of individual axon of neurons in the lateral geniculate nucleus (LGN) , anterograde tracing experiments using PHA-L were performed. The results clearly indicated that LGN axons extended to layr II-III outside the usual termination layr IV in the visual cortex treated with kainic acid. This abnormal termination pattern of LGN axon is considered as a reason why we could not obtain visual responses in the cortex injected with kainic acid. We also examined the effects of kainic acid inject … More ion on the development of the visual callosal projection. Retrograde tracing experiments have shown that the number of the callosal projection to the visual cortex injected with kainic acid was significantly reduced. Larger number of pyramidal cells were identified as callosal neurons in the contralateral visual cortex to the kainic acid injection. These results first demonstrated that the kainic acid injection in the first week of age affected not only thalamocortical projection but also the callosal projection in kitten visual cortex. It is necessary to examine the specificity of kainic acid injection on subplate cells, however, the present study first demonstrated that the interaction of axons with deep cortical cells, including subplate cells plays an important role for establishing appropriate contact to the post synaptic cells and maintaining them in the visual cortex We continue to study molecular and cellular mechanisms of formation of functional connections in the visual cortex during development. Less

亚板细胞的神经元活动和功能被认为与视皮层功能结构的形成有关。我们研究了海人酸视皮层局部注射对几天龄小猫的影响。电生理和免疫组织化学检查显示，这种药物操作阻止了正常视皮质功能的突触连接的发展。为了详细观察外侧膝状体神经元单个轴突的终末模式，用PHA-L进行了顺行示踪实验。结果清楚地表明，在红藻氨酸处理的视皮层中，LGN轴突延伸到通常的终末IV层之外的II-III层。这种异常的LGN轴突终末模式被认为是我们无法在注射海人酸的皮质获得视觉反应的原因之一。我们还观察了红藻氨酸注射…的效果。更多的离子作用于视觉胼胝体投射的发育。逆行追踪实验表明，注射红藻氨酸后，向视皮层的胼胝体投射数量明显减少。注射海人酸后，对侧视皮质内可见大量的锥体细胞为散在神经元。这些结果首次证明，1周龄注射红藻氨酸不仅影响丘脑皮质的投射，而且还影响猫视皮质的胼胝体投射。有必要研究注射海人酸对视皮质亚板细胞的特异性，然而，本研究首次证明轴突与深层皮质细胞(包括亚板细胞)的相互作用对于建立与突触后细胞的适当联系并维持它们在视觉皮质中的作用起着重要作用。我们继续研究发育过程中视觉皮质功能连接形成的分子和细胞机制。较少