OSMOREGULATORY MECHANISMS DURING EARLY LIFE STAGES IN FISH

鱼类生命早期阶段的渗透调节机制

• 批准号: 06839010
• 负责人: KANEKO Toyoji
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06839010/
• 关键词: Chloride cell; Yolk sac membrane; Osmoregulation; Chum salmon; Tilapia; 卵黄のう上皮; 発眼卵

The present study was undertaken to clarify the osmoregulatory mechanisms during early life stages in fish and to investigate the possible involvement of chloride cells in the yolk-sac membrane in osmoregulation, using euryhaline tilapia (Oreochromis mossambicus) and chum salmon (Oncorhynchus keta). The followings are the results obtained for the last two years :1.Eyed-stage embryos of chum salmon possess hypoosmoregulatory ability and chloride cells in the yolk-sac membrane may be involved in salt extrusion, in place of gill chloride cells, during the late embryonic stages.2.Similarly, in tilapia embryos, chloride cells in the yolk-sac membrane are potentially important sites for ion exchange in seawater.3.In tilapia, cortisol treatment stimulates the size and number of chloride cells in the yolk-sac membrane of embryos and larvae in vivo and in vitro, suggesting that cortisol present in the yolk may be involved in osmoregulation during early life stages.

本研究旨在阐明鱼类早期生命阶段的代谢调节机制，并探讨卵黄囊膜氯细胞参与代谢调节的可能性，使用广盐性罗非鱼（Oreochromis mossambicus）和鲑鱼（Oncorhynchus凯塔）。近两年的研究结果如下：1.鲑鱼眼期胚胎具有低盐调节能力，卵黄囊膜中的氯细胞可能代替鳃氯细胞参与胚胎晚期的盐分泌; 2.罗非鱼胚胎中，卵黄囊膜中的氯细胞是海水中离子交换的潜在重要位点; 3.在罗非鱼体内和体外，皮质醇处理刺激胚胎和幼体卵黄囊膜中氯细胞的大小和数量，表明卵黄中存在的皮质醇可能参与早期生命阶段的低盐调节。

项目成果

Ono, M,.Harigai, T., Kaneko, T., Sato, U., Ihara, S., and Kawauchi, H: "Pit-1/GH factor-1 involvement in the gene expression of somatolactin" Mol.Endocrinol. 8. 109-115 (1994)

Ono, M,.Harigai, T.、Kaneko, T.、Sato, U.、Ihara, S. 和 Kawauchi, H：“Pit-1/GH 因子 1 参与生长抑素基因表达”Mol.Endocrinol

Kakizawa,S.et al.: "Effects of feeding,fasting,background adaptation,acute stress,and exhaustive exercise on the plasma somatolactin concentrations" General Comparative Endocrinology. 98. 137-146 (1995)

Kakizawa,S.et al.：“进食、禁食、背景适应、急性应激和力竭运动对血浆生长激素浓度的影响”一般比较内分泌学。

Ayson, F.G., Kaneko, T., Hasegawa, S., and Hirano, T: "Defferential expression of two prolactin and growth hormone genes during early development of tilapia (Oreochromis mossambicus) in fresh water and seawater : Implications for possible involvement in o

Ayson, F.G.、Kaneko, T.、Hasekawa, S. 和 Hirano, T：“淡水和海水中罗非鱼 (Oreochromis mossambicus) 早期发育过程中两个催乳素和生长激素基因的差异表达：可能参与 o

Kakizawa, S., Kaneko, T., Ogasawara, T., Hirano, T: "Changes in plasma somatolactin levels during spawning migration of chum salmon (Oncorhynchus keta)" Fish Physiol.Biochem. 14. 93-101 (1995)

Kakizawa, S.、Kaneko, T.、Ogasawara, T.、Hirano, T：“鲑鱼（Oncorhynchus keta）产卵迁移过程中血浆生长激素水平的变化”Fish Physiol.Biochem。

Kakizawa,S.et al.: "Elevation of plasma somatolactin concentrations during acidosis in rainbow trout(Oncorhynchus mykiss)." Journal of Experimental Biology.(印刷中). (1996)

Kakizawa, S. 等人：“虹鳟鱼（Oncorhynchus mykiss）酸中毒时血浆生长激素浓度的升高。”实验生物学杂志（1996 年）。