Functional Diversity of Chloride Cells in Iono-and Osmo-Regulation in Fish
鱼类氯离子和渗透调节中氯细胞的功能多样性
基本信息
- 批准号:10660173
- 负责人:
- 金额:$ 2.62万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 1999
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1. To clarify the involvement of cortisol in functional differentiation of branchial chloride cells, cellular gene expression and localization of cortisol receptor were examined in chum salmon fry in fresh water (FW) and those adapted to seawater (SW) by jn situ hybridization and immunocytochemical staining. The receptor gene expression examined by in situ hybridization and specific immunostaining with anti-cortisol receptor serum were found in two types of chloride cells distributed in the gill filaments and lamellae, indicating that cortisol may be one of the important factors regulating chloride cell functions. Gene expression of cortisol receptor in filament chloride cells, which were highly activated in SW-adapted fry, was significantly greater in the fry adapted to SW than in FW-adapted fry. Cortisol receptors were also present in undifferentiated cells in the interlamellar regions, also suggesting the involvement of cortisol in the functional differentiation of chloride cells.2. Changes in chloride cell morphology were examined in the yolk-sac membrane of Mozambique tilapia embryos and larvae transferred from FW to seawater SW. Labeling chloride cells with DASPEI, a fluorescent probe specific for mitochondria, we observed jn vivo sequential changes in individual chloride cells by confocal laser scanning microscopy. In embryos transferred from FW to SW 3 days after fertilization, 75% of chloride cells survived 96 h later, and each cell showed a remarkable increase in size. In contrast, the cell size did not change in embryos and larvae kept in FW. The same rate of chloride cell turnover was observed in both FW and SW.Immunofluorescence observations revealed that single chloride cells enlarged and were frequently indented by newly-differentiated accessory cells to form multicellular complexes during SW adaptation. These results indicate that FW-type single chloride cells are transformed into SW-type multicellular complexes during SW adaptation.
1. 为了阐明皮质醇在鳃氯细胞功能分化中的作用,采用原位杂交和免疫细胞化学染色的方法检测了淡水鲑鱼(FW)和海水鲑鱼(SW)鱼苗中皮质醇受体的细胞基因表达和定位。原位杂交和抗皮质醇受体血清特异性免疫染色检测了分布在鳃丝和鳃片中的两种氯细胞的受体基因表达,提示皮质醇可能是调节氯细胞功能的重要因素之一。在SW适应的鱼苗中被高度激活的氯丝细胞中皮质醇受体的基因表达在SW适应的鱼苗中显著高于fw适应的鱼苗。皮质醇受体也存在于层间区未分化的细胞中,也提示皮质醇参与了氯细胞的功能分化。研究了莫桑比克罗非鱼胚和幼鱼卵黄囊膜中氯离子细胞形态的变化。我们用线粒体特异性荧光探针DASPEI标记氯细胞,通过共聚焦激光扫描显微镜观察单个氯细胞的体内序列变化。受精后3 d从FW转移到SW的胚胎,96 h后75%的氯细胞存活,且每个细胞的大小都显著增加。相反,在FW中保存的胚胎和幼虫细胞大小没有变化。在FW和SW中观察到相同的氯化物细胞更新速率。免疫荧光观察显示,在SW适应过程中,单个氯细胞扩大,并经常被新分化的辅助细胞压痕形成多细胞复合物。这些结果表明,在SW适应过程中,fw型单氯细胞转化为SW型多细胞复合物。
项目成果
期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Sasai, S.: "Morphological alteration in two types of gill chloride cells in Japanese eel (Anguilla japonica) during catadromous migration."Can. J. Zool.. 76. 1480-1487 (1998)
Sasai, S.:“日本鳗鱼(Anguilla japonica)在降迁过程中两种类型的鳃氯化物细胞的形态变化。”Can。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kaneko,T.: "Application of laser scanning microscopy for morphological observations on drinking in freshwater medaka and those exposed to 80% seawater"Fish. Sci.. 65. 492-493 (1999)
Kaneko,T.:%20"应用%20%20激光%20扫描%20显微镜%20用于%20形态%20观察%20on%20饮用%20in%20淡水%20medaka%20和%20那些%20暴露%20到%2080%%20海水"鱼。%
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sasai, S.: "Morphological alteration in two types of gill chloride cells in Japanese eel (Anguilla japonica) during catadromous migration"Can. J. Zool.. 76. 1480-1487 (1998)
Sasai, S.:“日本鳗鱼(Anguilla japonica)在降程迁移过程中两种类型的鳃氯化物细胞的形态变化”Can。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Uchida, K.: "Localization of cortisol receptor in branchial chloride cells in chum salmon fry."Gen. Comp. Endocrinol.. 109. 175-185 (1998)
Uchida, K.:“鲑鱼鱼苗鳃氯细胞中皮质醇受体的定位。”Gen.
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
N.Hirai: "Distributional changes in branchial chloride cells during fresh-water adaptation in Japanese sea bass." Zool.Sci.(印刷中). (1999)
N.Hirai:“日本鲈鱼淡水适应过程中鳃氯细胞的分布变化。”(正在出版)。
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KANEKO Toyoji其他文献
KANEKO Toyoji的其他文献
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{{ truncateString('KANEKO Toyoji', 18)}}的其他基金
Interaction of the gill, intestine and kidney and integration by endocrine systems in fish osmoregulation
鱼类渗透压调节中鳃、肠和肾的相互作用以及内分泌系统的整合
- 批准号:
19380109 - 财政年份:2007
- 资助金额:
$ 2.62万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Ion-transporting ability and functional differentiation of chloride cells in the yolk-sac membrane of"yolk balls"
“卵黄球”卵黄囊膜氯细胞的离子传输能力及功能分化
- 批准号:
14360106 - 财政年份:2002
- 资助金额:
$ 2.62万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Adaptation to various aquatic environments and functional diversity of chloride cells in Fish
鱼类对各种水生环境的适应及氯细胞功能多样性
- 批准号:
12660163 - 财政年份:2000
- 资助金额:
$ 2.62万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Ion Regulatory Mechanisms of Chloride Cells in Chum Salmon
鲑鱼氯离子细胞的离子调节机制
- 批准号:
08833006 - 财政年份:1996
- 资助金额:
$ 2.62万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
OSMOREGULATORY MECHANISMS DURING EARLY LIFE STAGES IN FISH
鱼类生命早期阶段的渗透调节机制
- 批准号:
06839010 - 财政年份:1994
- 资助金额:
$ 2.62万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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