PPR proteins as modulators of chloroplast translation and RNA stability during temperature acclimation (A07)

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The PPR proteins SVR7 and PGR3 are important ribosome biogenesis factors and MRL1 is a PPR required for Rubisco expression. We found that all three PPR genes are induced during cold acclimation and that in general, ribosome biogenesis factors and ribosomal components are cold-regulated. Our goal is to understand how the selected PPR proteins influence RNA stability and translation of their target mRNAs during temperature acclimation. Furthermore, we will investigate by super resolution microscopy, where ribosome biogenesis takes place in chloroplasts and will use quantitative biochemistry to determine how ribosome numbers change in response to temperature acclimation.