PPR proteins as modulators of chloroplast translation and RNA stability during temperature acclimation (A07)

PPR 蛋白作为温度驯化过程中叶绿体翻译和 RNA 稳定性的调节剂 (A07)

• 批准号: 444905138
• 金额: --
• 依托单位: Institut für Biologie
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/444905138?language=en
• 关键词: PPR; proteins; modulators; chloroplast; translation

The PPR proteins SVR7 and PGR3 are important ribosome biogenesis factors and MRL1 is a PPR required for Rubisco expression. We found that all three PPR genes are induced during cold acclimation and that in general, ribosome biogenesis factors and ribosomal components are cold-regulated. Our goal is to understand how the selected PPR proteins influence RNA stability and translation of their target mRNAs during temperature acclimation. Furthermore, we will investigate by super resolution microscopy, where ribosome biogenesis takes place in chloroplasts and will use quantitative biochemistry to determine how ribosome numbers change in response to temperature acclimation.

PPR蛋白SVR7和PGR 3是重要的核糖体生物合成因子，MRL 1是Rubisco表达所需的PPR。我们发现，所有这三个PPR基因的诱导过程中冷驯化，并在一般情况下，核糖体生物合成因子和核糖体成分的冷调节。我们的目标是了解所选的PPR蛋白如何影响RNA稳定性和在温度适应期间其靶mRNA的翻译。此外，我们将调查超分辨率显微镜，核糖体生物发生在叶绿体中，并将使用定量生物化学来确定核糖体数量如何响应温度驯化的变化。