High throughput cell separation device

高通量细胞分离装置

• 批准号: 447860429
• 金额: --
• 依托单位: Julius-Maximilians-Universität Würzburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/447860429?language=en
• 关键词: throughput; cell; separation; device

Currently, the separation of cells and cell subsets is usually performed by a magnetic system with manual purification. The cells are labelled with specific antibodies coupled with magnetic particles, trapped in a magnetic field and then rinsed out again at high pressure. This procedure is associated with a high stress potential for the cells and is particularly toxic for sensitive cell types such as human stem cells. An alternative is purification using a cell sorter in the adjacent Core Facility. However, this method is not sterile and involves a high risk of contamination. The device applied for, on in contrast, is capable of very gently purifying complex cell populations by means of the expression of surface markers. This purification is carried out under low pressure, without compression or alteration of the electrical charge. The purity of the sorted cells usually reaches ≥ 95% after separation The process is completely sterile, since it works in a completely closed system, so that cells can be kept without the addition of antibiotics. Furthermore, the separation of the cell populations after initial setup is fully automated, which results in a significant time saving.

目前，细胞和细胞亚群的分离通常是通过磁性系统进行手动纯化。这些细胞用特异性抗体和磁性颗粒标记，被困在磁场中，然后再次在高压下冲洗。该过程与细胞的高应激潜力相关，并且对敏感细胞类型如人类干细胞特别有毒。另一种选择是使用邻近核心设施中的细胞分选机进行纯化。然而，这种方法不是无菌的，并且具有很高的污染风险。相比之下，所申请的装置能够通过表达表面标志物非常温和地纯化复杂的细胞群。这种纯化在低压下进行，不压缩或改变电荷。分离后分选细胞的纯度通常达到≥ 95%该过程是完全无菌的，因为它在完全封闭的系统中工作，因此可以在不添加抗生素的情况下保存细胞。此外，在初始设置后，细胞群的分离是完全自动化的，这大大节省了时间。