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Creation of artificial cell wall by mimicking the process of natural cell wall formation

通过模仿天然细胞壁形成过程来创建人造细胞壁

基本信息

批准号：
15208016
负责人：
URAKI Yasumitsu
金额：
$ 31.12万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

An aim of this research is to clarify the construction mechanism of tree cell wall that had the hierarchical structure from molecular level to tissue level. In addition, our objective is to create an artificial cell wall by mimicking the formation process of natural wood cell wall on the basis of the results of former subjects. In this research project, we obtained experimental results and conclusions for five subjects.Conclusion 1.Xylan is a key hemicellulose for lignification.Conclusion 2.This finding for ConClusion 1 was supported by physicochemical analysis of the interaction between hemicelluloses and lignification-related compounds. These analyses were carried out on an apparatus based on the surface plasmon resonance.Conclusion 3.We clarified solution behavior of LCC in water, such as interaction of solvent, self-assembly of LCC molecules and formation of hydrophobic domain in the self-aggregates. We proposed LCC functions in the wood from these results.Conclusion 4.we developed a new analysis method, Time of Flight・Secondary Ion Mass spectroscopy (ToF-SIMS), for lignin structure, and artificial lignin polymer, which was merely comprised of β-O-4, as a lignin model for NMR assignment.Conclusion 4 We elucidate the cell wall formation at the cell level in relation to cell-supported proteins, microtubles and actin in addition to tissue formation.Conclusion 5.we developed a methodology to create artificial cell wall on the basis of findings obtained in this study.The artificial cell wall backbone was fabricated by the conversion of bacterial cellulose to honeycomb-patterned material. The conversion was carried out by the control of the movement of cellulose-producing bacteria.

本研究旨在阐明树木细胞壁从分子水平到组织水平具有层次结构的构建机制。此外，我们的目标是在前人研究成果的基础上，模拟天然木材细胞壁的形成过程，构建人工细胞壁。本研究通过对5个课题的实验研究，获得了以下结果和结论：1.木聚糖是木质化过程中的关键半纤维素。结论3.阐明了LCC在水溶液中的溶解行为，如溶剂的相互作用、LCC分子的自组装以及自聚集体中疏水结构域的形成。结论4.建立了飞行时间·二次离子质谱分析方法（ToF-SIMS），以及人工合成的木质素聚合物（仅含β-O-4）作为木质素的NMR模型。结论4我们从细胞水平上阐明了细胞壁形成与细胞支持蛋白的关系，结论5.在本研究的基础上，我们开发了一种人工细胞壁的方法，通过将细菌纤维素转化为蜂窝状材料来制备人工细胞壁骨架。通过控制产纤维素细菌的运动进行转化。