Development of Novel Hybrid Bone Substitute with Stem cells and Growth Factors

我们采用“形式-凝胶”技术制备了一种完全互联的多孔羟基磷灰石(IP-CHA)。这种相互连接的多孔结构允许将骨细胞、重组人骨形态发生蛋白-2或血管系统引入毛孔，从而促进了组织工程。IP-CHA与大鼠骨髓间充质细胞结合后，在孔洞内可见丰富的骨形成。IP-CHA复合重组人骨形态发生蛋白-2(rhBMP-2)可完全修复兔桡骨的骨缺损。植入上腹壁血管的IP-CHA/BMP复合材料在靠近插入血管的深部小孔中有大量的新骨形成。IP-CHA的三维完全互连的多孔结构促进了材料中的骨生长，最终导致材料良好地结合到宿主骨中。合成的支架可以预制成特定的尺寸和形状以匹配骨缺损，甚至可以预制成含有固体羟基磷灰石的复合材料，以增强其初始机械强度。

We developed a fully interconnected porous hydroxyapatite (IP-CHA) by adopting the "form-gel" technique. The interconnected porous structure facilitates tissue engineering by allowing the introduction of bone cells, rhBMP-2, or vasculature into the pores. IP-CHA combined with rat marrow mesenchymal cells showed abundant bone formation inside the pore areas. IP-CHA combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) completely repaired the bony defect of rabbit radius. IP-CHA/BMP composite with insertion of epigastric vessels exhibited abundant new bone formation in the pores of the deep portion close to the inserted vessels. The 3-dimensional fully interconnected porous structure of IP-CHA encourages bone ingrowth in the material and eventually leads to good incorporation of the material into host bone. The synthetic scaffold can be prefabricated into specific sizes and shapes to match bone defects, and even into composite with solid form of HA in order to reinforce its initial mechanical strength.