Development of Myocardial Regenerative Therapy using Highly Regular Honeycomb-Patterned Scaffold inducing tissue self-regeneration

细胞治疗心肌梗死后心肌修复是一种很有前景的新方法。然而，细胞治疗通常需要长时间培养细胞，以增加足够数量的细胞。为了解决这一问题，需要使用合适的支架来实现细胞附着、增殖、分化和多种功能。在这项研究中，我们通过简单的铸造技术，评估了细胞表面形貌对心肌细胞治疗行为的影响。与平面膜相比，蜂窝状膜能显著促进骨髓间充质干细胞和成肌细胞的增殖。在拉伸蜂窝状膜上，心肌细胞形态与拉伸方向正确对齐，而在正常膜上不对齐。在体内观察到蜂窝状膜的再细胞化。高度规整的膜具有可控制的孔径和表面形貌，对细胞的增殖、分化和排列有很强的影响。蜂窝状膜可能是心肌再生治疗支架的新候选材料

Cell therapy for myocardial repair after myocardial infarction is a new and promising strategy. However, cell therapy generally needs to culture the cell for a long period in order to increase a sufficient number of cells. To solve this problem, it needs to use a suitable scaffold for cell attachment, proliferation, differentiation and several functions. In this study, we evaluated the effect of surface topography in the cell for myocardial cell therapy behavior using the highly regular patterned biodegradable films by simple casting technique.The bon marrow-derived mesenchymal stem cell and myoblast proliferation was significantly accelerated on the honeycomb-patterned film as compared to the flat films. On the stretched honeycomb-patterned films, the cardiac cell morphology was correctly aligned to the stretch direction and not aligned on the normal films. Recellularization of honeycomb-patterned film was observed in vivo.The highly regular patterned films with controlled pore size and surface topography have a strong effect on the cell proliferation differentiation and alignment. The honeycomb-patterned films may be a novel candidate of the scaffold for Myocardial Regenerative Therapy