Development of Myocardial Regenerative Therapy using Highly Regular Honeycomb-Patterned Scaffold inducing tissue self-regeneration

使用高度规则的蜂窝图案支架诱导组织自我再生开发心肌再生疗法

• 批准号: 16209042
• 负责人: SAWA Yoshiki
• 金额: $ 25.79万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16209042/
• 关键词: honeycomb-patterned film; scaffold; Recellularization; Myocardial Regenerative; ナノハニカムシート; 心筋再生治療; 生分解性多孔性足場; 生分解性多孔体足場; 心筋再生治療法

Cell therapy for myocardial repair after myocardial infarction is a new and promising strategy. However, cell therapy generally needs to culture the cell for a long period in order to increase a sufficient number of cells. To solve this problem, it needs to use a suitable scaffold for cell attachment, proliferation, differentiation and several functions. In this study, we evaluated the effect of surface topography in the cell for myocardial cell therapy behavior using the highly regular patterned biodegradable films by simple casting technique.The bon marrow-derived mesenchymal stem cell and myoblast proliferation was significantly accelerated on the honeycomb-patterned film as compared to the flat films. On the stretched honeycomb-patterned films, the cardiac cell morphology was correctly aligned to the stretch direction and not aligned on the normal films. Recellularization of honeycomb-patterned film was observed in vivo.The highly regular patterned films with controlled pore size and surface topography have a strong effect on the cell proliferation differentiation and alignment. The honeycomb-patterned films may be a novel candidate of the scaffold for Myocardial Regenerative Therapy

细胞治疗心肌梗死后心肌修复是一个新的和有前途的策略。然而，细胞疗法通常需要长时间培养细胞以增加足够数量的细胞。为了解决这个问题，需要使用合适的支架来实现细胞的附着、增殖、分化和多种功能。在这项研究中，我们评估了细胞表面形貌对心肌细胞治疗行为的影响，使用高度规则的图案化生物可降解薄膜，通过简单的铸造技术，骨髓间充质干细胞和成肌细胞增殖显着加速蜂窝图案的薄膜相比，平坦的薄膜。在拉伸的蜂窝状图案的膜上，心脏细胞形态正确地与拉伸方向对齐，而在正常膜上不对齐。在体内观察到蜂窝状图案化膜的再细胞化，具有可控孔径和表面形貌的高度规则图案化膜对细胞的增殖、分化和排列有很强的影响。蜂窝状薄膜有望成为心肌再生治疗支架材料