Multilabel lipid tracing: deciphering hepatocyte lipid metabolism with time- and species resolution

多标记脂质追踪：以时间和物种分辨率破译肝细胞脂质代谢

• 批准号: 450419533
• 负责人: Professor Dr. Christoph Thiele
• 金额: --
• 依托单位: Abteilung Biochemie & Zellbiologie der Lipide
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/450419533?language=en
• 关键词: Multilabel; lipid; tracing; deciphering; hepatocyte

Hepatocyte lipid metabolism does not only determine the lipid status of the liver itself, but is the major regulator of whole body lipid status and turnover with major implications in many types of disease. Due to experimental limitations in sensitivity and time resolution, our understanding of the dynamics of hepatic lipid metabolism is quite limited. As detailed below, we recently introduced new technology that offers strongly improved sensitivity and time resolution. This application describes further development of the technology and its use for better understanding the dynamics of hepatocyte lipid metabolism. The focus of the work will be on the two quantitatively major lipid classes, i.e. phosphatidylcholine and triacylglycerol.

肝细胞脂代谢不仅决定肝脏本身的脂状态，而且是全身脂状态和周转的主要调节因素，在许多类型的疾病中具有重要意义。由于灵敏度和时间分辨率的实验限制，我们对肝脂代谢动力学的了解相当有限。如下所述，我们最近推出了新技术，大大提高了灵敏度和时间分辨率。本申请描述了该技术的进一步发展及其应用，以更好地了解肝细胞脂质代谢的动力学。工作的重点将集中在两种主要的定量类脂上，即磷脂酰胆碱和三酰甘油。