Fabrication of Novel Biointerfaces Using High-Density Polymer Brushes

使用高密度聚合物刷制造新型生物界面

• 批准号: 17205022
• 负责人: TSUJII Yoshinobu
• 金额: $ 31.7万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17205022/
• 关键词: biointerface; polymer brush; concentrated brush; living radical polymerization; surface modification; protein adsorption; cell adhesion; tribology; ポリマーブラン; 末端機能化; グラフト重合; 水溶性ポリマー; 潤滑; 脂質膜; コンビナトリアル手法

Living radical polymerization has been applied to surface-initiated graft polymerization, allowing controlled grafting of well-defined polymers from various solid surfaces with dramatically high surface densities. This density was more than 1 order of magnitude higher than those of typical semidilute brushes, going deep into the concentrated brush regime. This project aimed at clarifying the properties of concentrated polymer brushes as novel biointerfaces. The main results are as follows.(1) The protein adsorption as well as the cell adhesion could be much reduced on the concentrated brushes of hydrophilic polymers because of their size-exclusion effect and dynamic properties. This excellent inertness of the concentrated brush in the interaction with the proteins should afford the system long-term stability against biofauling.(2) Photo-induced organotellurium-mediated living radical polymerization successfully applied to the preparation of the hydrophilic brushes.(3) Combinatorial technique was established to fabricate a brush surface with continuous variation of graft density and chain length, clarifying the crossover density of concentrated/semi-dilute brushes.(4) An excellent frictional property was macroscopically demonstrated fix the system of a concentrated-brush and gel surfaces.(5) The concentrated brushes of poly (N-isopropylacrylamide) were synthesized and studied on their properties of swelling/deswelling, friction, and protein adhesion.(6) A new and novel technique was established to immobilize initiators on polymeric substrates as well as various metal surfaces.

活性自由基聚合已被应用于表面引发的接枝聚合，允许从具有显着高表面密度的各种固体表面控制接枝定义明确的聚合物。这种密度比典型的半稀释刷高1个数量级以上，深入到集中刷政权。该项目旨在阐明浓缩聚合物刷作为新型生物界面的性质。主要结果如下。(1)由于亲水性聚合物的尺寸排阻效应和动力学特性，蛋白质吸附和细胞粘附在亲水性聚合物的浓缩刷上可以大大减少。浓缩刷在与蛋白质的相互作用中的这种优异的惰性应该为系统提供针对生物故障的长期稳定性。(2)光诱导有机碲介导的活性自由基聚合成功地应用于亲水刷的制备。(3)建立了一种组合技术来制备具有连续变化的接枝密度和链长的刷表面，澄清了浓/半稀刷的交叉密度。(4)宏观上证明了集中刷和凝胶表面的固定系统具有优异的摩擦性能。(5)合成了聚（N-异丙基丙烯酰胺）浓缩刷，并对其溶胀/去溶胀、摩擦和蛋白质粘附性能进行了研究。(6)建立了一种新的和新颖的技术，将引发剂分散在聚合物基底以及各种金属表面上。

项目成果

Size-exclusion Effect and Protein Repellency of Concentrated Polymer Brushes Prepared by Surface-initiated Living Radical Polymerization

表面引发活性自由基聚合制备浓缩聚合物刷的尺寸排阻效应和蛋白质排斥性

• 发表时间: 2007
• 期刊: Macromol. Symp 248
• 作者: Y.;Kusaka;S.;Nakamura;K.;Azuma;T.;Sasaki;T.;Unate;Y.;Nakatani;A.;Nakasuga;K.;Matsukawa;N.;Watanabe;H.;Naito;H.;Kaji;C. Yoshikawa
• 通讯作者: C. Yoshikawa

界面に注目した新しい医用材料開発

以界面为重点的新型医用材料开发

• 发表时间: 2007
• 期刊: バイオマテリアル 25
• 作者: Marina Kotani;Hidehiko Dohi;Hideaki Kimura;Kiyoshige Muraoka;and Hironori Kaji;岸田晶夫
• 通讯作者: 岸田晶夫

Protein Repellency of Well-Defined, Concentrated Poly(2-hydroxyethl methacrylate) Brushes by Size-Exclusion Effect

通过尺寸排阻效应确定明确的浓缩聚（甲基丙烯酸 2-羟基乙酯）刷的蛋白质排斥性

• 发表时间: 2006
• 期刊: Macromolecules 39
• 作者: T.Fujigaya;S.Haraguchi;T.Fukumaru;N.Nakashima;C.Yoshikawa et al.
• 通讯作者: C.Yoshikawa et al.

リビングラジカル重合法による精密表面改質

采用活性自由基聚合法进行精密表面改性

• 发表时间: 2008
• 作者: M.Shigeta;M.Komatsu;N.Nakashima;辻井敬亘
• 通讯作者: 辻井敬亘

新しい表面特性を生み出す"濃厚"ポリマーブラシ

“厚”聚合物刷创造新的表面特性

• 发表时间: 2007
• 作者: Hironori;Kaji;田中泰彦;Y. Tsujii;辻井敬亘
• 通讯作者: 辻井敬亘