Study on the structure and funciotion of supramolecular flagellar motor

超分子鞭毛马达的结构与功能研究

• 批准号: 18207011
• 负责人: HOMMA Michio
• 金额: $ 31.45万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18207011/
• 关键词: べん毛; エネルギー変換; メンブレン; ナノバイオ; 分子モーター

The proteins, PomA, PomB, MotX and MotY have been identified as the motor proteins that are necessary for the flagella rotation. The T ring newly found by us is necessary for the assembly. The structure of the component protein, MotY, of the T ring is determined with a 2.9 Å resolution. We proposed the molecule dynamic model of the flagella motor proteins to assemble as a functional motor. We showed that the C ring structure was easily dissociated from the flagella base body. Furthermore we found that FlgT was the basal body component to construct a ring outside of the LP ring. The ring was named an H ring. The new ring may contribute on the Vibro motor function as a strong shaft to support the high-speed rotation of the motor.

PomA、PomB、MotX和MotY蛋白已被鉴定为鞭毛旋转所必需的马达蛋白。我们新发现的T形环是装配所必需的。T环的组分蛋白MotY的结构以2.9 μ m的分辨率测定。提出了鞭毛马达蛋白组装成功能马达的分子动力学模型。我们发现C环结构很容易从鞭毛基体上分离出来。此外，我们发现FlgT是在LP环外构建环的基体成分。这枚戒指被命名为H环。新的环可能有助于Vibro电机功能，作为一个强大的轴，以支持电机的高速旋转。

项目成果

Cell-free synthesis of the torque-generating membrane proteins, PomA and PomB, of the Nat-driven flagellar motor in Vibrio alginolyticus.

溶藻弧菌 Nat 驱动的鞭毛马达的扭矩产生膜蛋白 PomA 和 PomB 的无细胞合成。

• 发表时间: 2008
• 期刊: J. Biochem. 144(5)
• 作者: Terashima;H.;Abe-Yoshizumi;R.;Kojima;S.;Homma;M.
• 通讯作者: M.

In vivo架橋を用いた大腸菌走化性における受容体クラスターの構造機能連関の解析

利用体内交联分析大肠杆菌趋化性受体簇的结构-功能关系

• 发表时间: 2007
• 作者: M. Isobe;M. Kurono;K. Tsuboi< and A. Takai;入枝泰樹
• 通讯作者: 入枝泰樹

Cell-free synthesis of the torque-generating membrane proteins

扭矩产生膜蛋白的无细胞合成

• 期刊: PomA and PomB, of the Na^+-driven flagellar motor in Vibrio alginolyticus. J Biochem 144
• 作者: Terashima H;Abe-Yoshizumi R;Kojima S;Homma M.
• 通讯作者: Homma M.

Roles of charged residues of rotor and stator in flagellar rotation : Comparative study using H^+-driven and N^+-driven motors in Escherichia coli.

转子和定子带电残基在鞭毛旋转中的作用：在大肠杆菌中使用 H^ 驱动和 N^ 驱动电机的比较研究。

• 发表时间: 2006
• 期刊: J. Bacteriol. 188
• 作者: Yakushi;T.;Yang;J.-H.;Fukuoka;H.;Homma;M.;Blair D.
• 通讯作者: Blair D.

Stator assembly and activation mechanism of the flagellar motor by the periplasmic region of MotB

• DOI: 10.1111/j.1365-2958.2009.06802.x
• 发表时间: 2009-08-01
• 期刊: MOLECULAR MICROBIOLOGY
• 影响因子: 3.6
• 作者: Kojima, Seiji;Imada, Katsumi;Namba, Keiichi
• 通讯作者: Namba, Keiichi