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Roles of the chemotaxis and the motility by flagella on environmental response of Vibio cells

鞭毛趋化性和运动性对Vibio细胞环境反应的作用

基本信息

批准号：
16017241
负责人：
HOMMA Michio
金额：
$ 9.41万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16017241/
关键词：
Chemotaxis Signal transduction Receptor Flagella Vibrio

项目摘要

Vibrio cholerae has 3 sets of general chemotactic signaling proteins (Che proteins) and 45 chemoreceptor-like proteins (referred to as MLPs). Systems I cluster and III cluster in the chromosome have 4 mlp genes and 2 mlp genes, respectively, and we speculated that those genes were coupled CheAl and CheA3. System II cluster does not have mlp genes. To estimate the mlp genes coupled to any of the three Che systems, the primary sequences were analyzed and 19 MLPs have a similarity to MLPs of V. parahemolyticas which have only one CheA homologous to CheA2 of V. *cholerae. The 19 MLPs are candidates to couple with CheA2. To evaluate the assumption, the methylation of MLP by the homologous product of the methyl transfer enzyme CheR was examined. The similar motif as the CheR binding sequence of E. coli MCP was found in the C terminal region of M1p01 and M1p45, and CheR3 has the highest similarity to E. coli CheR among the three of the CheR homologs. This motif might give a binding site of CheR2 and is important for metylation of MLP coupled to the putative CheA2. Next, we examined the localization of Che system components by using their GFP fusion constructs. We detected that the Che proteins and MLPs of system II localize to the flagellated pole and some of the components of systems I were also shown to localize to the flagellated pole. During these observations, we noticed that the system I components were diffused under some conditions. When cells were grown to late logarithmic phase with vigorous shaking, CheAl and CheY2 did not localize but further incubation without shaking induced their polar localization. Moreover, when the air was replaced with nitrogen gas, shaking allowed their polar localization. Inhibitors of respiration also induced their polar localization. These results indicate that certain state of energy metabolism induces polar localization of CheAl and CheY2, raising the possibility that system I may work under microanaerobic conditions.

霍乱弧菌具有3组一般趋化性信号蛋白（Che蛋白）和45种化学受体样蛋白（称为MLP）。染色体上的系统I簇和系统III簇分别具有4个mlp基因和2个mlp基因，我们推测这些基因是CheA1和CheA3偶联的。系统II簇不具有mlp基因。为了估计与三种Che系统中的任一种偶联的mlp基因，分析了一级序列，并且19个MLP与副溶血性弧菌的MLP具有相似性，副溶血性弧菌的MLP仅具有一个与霍乱弧菌的CheA2同源的CheA。这19个MLP是与CheA2配对的候选者。为了评估假设，通过甲基转移酶CheR的同源产物检查MLP的甲基化。与E.在M1p01和M1p45的C端区域发现了与大肠杆菌MCP的同源性，其中与大肠杆菌MCP同源性最高的是CheR3。coliCheR同源物中的一个。该基序可能提供CheR2的结合位点，并且对于与推定的CheA2偶联的MLP的甲基化是重要的。接下来，我们通过使用它们的GFP融合构建体来检查Che系统组件的定位。我们检测到，车蛋白和MLP的系统II本地化的鞭毛虫杆和系统I的一些组件也被证明本地化的鞭毛虫杆。在这些观测中，我们注意到系统I的成分在某些条件下是扩散的。当细胞在剧烈振荡下生长至对数生长期晚期时，CheAl和CheY2不定位，但在不振荡的情况下进一步孵育诱导其极性定位。此外，当用氮气代替空气时，摇动使它们的极性定位。呼吸抑制剂也诱导其极性定位。这些结果表明，某些状态的能量代谢诱导的极性本地化的CheAl和CheY2，提高了系统I可能在微厌氧条件下工作的可能性。

项目成果

期刊论文数量（34）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Attractant binding alters arrangement of chemoreceptor dimers within its cluster at a cell pole

DOI：
10.1073/pnas.0306660101
发表时间：
2004-03-09
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Homma, M;Shiomi, D;Kawagishi, I
通讯作者：
Kawagishi, I

Roles of charged residues of rotor and stator in flagellar rotation : Comparative study using H^+-driven and N^+-driven motors in Escherichia coli.

转子和定子带电残基在鞭毛旋转中的作用：在大肠杆菌中使用 H^ 驱动和 N^ 驱动电机的比较研究。