Basic studies on development of alternative fuels using hydrocarbon-producing algae.

通过对天然环境中烃类合成途径、调控机制和芽孢球菌爆发等生物化学方面的研究，为开发石油替代资源提供必要的知识。有人建议：2) 63株芽孢球菌产生的碳氢化合物结构可分为正烯（A种，6%）、三萜（B种，59%）、四萜（L种，5%）和其他（30%），3)菌株bot70通过MEP途径合成萜类油，4)激活SAM循环，提供SAM合成芽孢杆菌，5)照射。热分层和氮源浓度是芽孢球菌占优势的重要因素，40μmol光子/m^2/s以上的多余能量被用于生产碳氢化合物而不是用于细胞生长。

Bilogical and chemical studies related to synthetic pathway and regulatory mechanisms of hydrocarbons and outbreak of the Botryococcus bloom in natural environments were investigated to obtain the knowledges essential for the development of alternative resource of petroleum. It was suugested that : 1) cell and colony shapes and oil production were greatly influenced by iron compounds, 2) The elucidated structures of hydrocarbons produced by 63 strains of Botryococcus could be classified n-alekene (Race A, 6%), triterpene (Race B, 59%),tetraterpene (Race L, 5%) and others (30%), 3) the strain BOT-70 synthesized the terpenoid oils by MEP pathway, 4) the SAM cycle was activated to supply SAM to synthesize botryococcene, 5) irradiation, thermal stratification and concentration of nitrogen sources are important factors for predominancy of Botryococcus and 6) excess energy above 40μmol photons/m^2/s was used for the production of hydrocarbons instead of that of cell growth.