Development of a novel detection method for abnormal prion protein

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The aim of this research is to develop the novel detection method for the abnormal prion protein, which causes the prion disease. The recombinant normal prion protein was expressed in E. coli, and purified with affinity chromatography. The denatured prion protein, which indicates resistance against protease digestion, was prepared by the treatment of the normal prion protein with cupric ions. In this project, we developed highly sensitive and specific detection methods for the prion proteins on a solid phase membrane using nucleic aptamer and chemiluminescent reagents.