Development of the discrimination method for mutation and drug resistance of an AIDS virus

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Acquired immunodeficiency syndrome (AIDS) is caused by HIV infection, and one of the most serious infectious diseases. Moreover, the emergence and transmission of drug resistance strains of HIV are the most serious problem in the world. Thus, new treatments and discrimination methods for drug resistance HIV are still in great need.A noble assay method for HIV protease activity was developed, basing on a specific fluorescence derivatization reaction for peptides, previously. In this research, I tried to apply this HIV protease assay to discrimination for drug-resistant HIV proteases.Consequently, mutant HIV proteases with drug-resistant could be directly discriminated by the present discrimination method.