Measurement and control for multiscale-spatiotemporal neurodynamics

本课题对多尺度时空神经动力学、三位数空间和十位数时间的测量和控制提出了挑战。该项目的目的如下：多电极阵列长期培养，脉冲检测，神经网络生长方式观察，所得数据分析。因此，我们在多电极阵列上建立了长期的原代神经元分离细胞培养。此外，在几个月的时间里，还对神经元主要部位的一些蛋白质进行了免疫细胞化学检测。完成了构建实验系统以解决神经科学基本问题的主要任务。利用构造的系统，得到了相关领域的重要问题。其中一个结果是，导致同步爆发的存活神经元的最小密度。本研究的进一步发展有望为未来在时空神经动力学方面的富有成效的研究奠定基础。

This project has challenged to measurement and control of multi-scale spatiotemporal neuronal dynamics, three-figure spatially and ten-figure temporally. The aim of the project is as follows ; long term culture on multi-electrode array, detection of impulses, observation of way of growing neuronal networks, and analysis of obtained data. As a result, we established long term primary neuronal dissociative cell culture on multi-electrode arrays. Moreover, immunocytochemistry of some proteins was also established for main parts of neuron during several month. The main subject of construction of experimental systems to attack basic problems in neuroscience was achieved. Using constructed system, we obtained important issues of the related field. One of the results is a minimum density of surviving neurons that caused synchronized bursts. Further development of the present works is expected to execute the future fruitful studies on spatiotemporal neuronal dynamics.