Measurement and control for multiscale-spatiotemporal neurodynamics

该项目对多尺度时空神经元动力学、空间三位数和时间十位数的测量和控制提出了挑战。该项目的目标是：在多电极阵列上进行长期培养，检测脉冲，观察神经元网络的生长方式，并对获得的数据进行分析。因此，我们在多电极阵列上建立了长期的原代神经元分离细胞培养。此外，在几个月的时间里，神经元的主要部分也建立了一些蛋白质的免疫细胞化学。实现了神经科学基本问题实验体系建设的主要课题。利用所构建的系统，我们得到了相关领域的重要问题。结果之一是导致同步爆发的存活神经元的最小密度。本工作的进一步发展有望推动未来时空神经元动力学的研究取得丰硕成果。

This project has challenged to measurement and control of multi-scale spatiotemporal neuronal dynamics, three-figure spatially and ten-figure temporally. The aim of the project is as follows ; long term culture on multi-electrode array, detection of impulses, observation of way of growing neuronal networks, and analysis of obtained data. As a result, we established long term primary neuronal dissociative cell culture on multi-electrode arrays. Moreover, immunocytochemistry of some proteins was also established for main parts of neuron during several month. The main subject of construction of experimental systems to attack basic problems in neuroscience was achieved. Using constructed system, we obtained important issues of the related field. One of the results is a minimum density of surviving neurons that caused synchronized bursts. Further development of the present works is expected to execute the future fruitful studies on spatiotemporal neuronal dynamics.