Relationship of cytoplasmic capping to translation and cell stress

本研究的目的是验证加帽在细胞质和细胞核中都可以发生，并揭示细胞质加帽在基因表达中的意义。我鉴定了许多靶mRNA，帽结构将在细胞质中添加。此外，深度测序分析表明，这些mRNA中的一些可能在3'末端具有聚（U）尾。这些修饰可能在基因表达中起重要作用。在另一个研究项目中，我发现T4噬菌体有一种抗大肠杆菌毒素的抗毒素，以使其自身生存。

The purpose of this research project is to verify that capping can occur in cytoplasm as well as nucleus, and show the significance of cytoplasmic capping in gene expression. I identified many target mRNAs to which cap structures would be added in cytoplasm. Also, deep sequencing analysis suggested that some of these mRNAs might have poly(U) tail at 3' terminus. These modifications on mRNA may play important roles in gene expression. In a different research project, I showed that bacteriophage T4 had an antitoxin against E.coli toxins for its own survival.