Analysis of the role of Notch signal of NK cell in hematopoietic stem cell transplantation

我们的研究目的是阐明NK细胞Notch信号在造血干细胞移植后移植物抗宿主病（GVHD）中的作用。用Notch配体刺激人脐带血cd34阳性细胞诱导功能成熟NK细胞。建立同种异体干细胞移植GVHD模型。当缺口诱导的NK细胞输注到受体中时，观察到GVHD的减少。研究发现，造血干细胞移植后患者的NK细胞表达Notch1，作为GVHD靶点的肠上皮细胞表达Notch配体Jagged1。提示NK细胞Notch信号与GVHD的关系。

The purpose of our study is to clarify roles of Notch signal of NK cell in graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation. Functionally mature NK cells were induced from human cord-blood CD34-positive cells by stimulation with Notch ligand. We established allogeneic stem cell transplantation GVHD model. Diminishment of GVHD was observed when Notch-induced NK cells were transfused into the recipient. It is revealed that NK cells of patients after hematopoietic stem-cell transplantation expressed Notch1 and intestinal epithelial cells, which are targets of GVHD, expressed Notch ligand, Jagged1. This result suggests the relation between Notch signal of NK cell and GVHD.