Analysis of the role of Notch signal of NK cell in hematopoietic stem cell transplantation

NK细胞Notch信号在造血干细胞移植中的作用分析

• 批准号: 21890027
• 负责人: KURITA Naoki
• 金额: $ 1.66万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21890027/
• 关键词: Notch1; GVHD; NK細胞; 造血幹細胞移植; 移植片対宿主病; Notchシグナル

The purpose of our study is to clarify roles of Notch signal of NK cell in graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation. Functionally mature NK cells were induced from human cord-blood CD34-positive cells by stimulation with Notch ligand. We established allogeneic stem cell transplantation GVHD model. Diminishment of GVHD was observed when Notch-induced NK cells were transfused into the recipient. It is revealed that NK cells of patients after hematopoietic stem-cell transplantation expressed Notch1 and intestinal epithelial cells, which are targets of GVHD, expressed Notch ligand, Jagged1. This result suggests the relation between Notch signal of NK cell and GVHD.

本研究的目的是阐明NK细胞Notch信号在造血干细胞移植后移植物抗宿主病（GVHD）中的作用。通过Notch配体刺激，从人脐带血CD34阳性细胞诱导出功能成熟的NK细胞。我们建立了异基因干细胞移植GVHD模型。当将 Notch 诱导的 NK 细胞输注至受体体内时，观察到 GVHD 减少。研究表明，造血干细胞移植后患者的NK细胞表达Notch1，GVHD靶标肠上皮细胞表达Notch配体Jagged1。该结果提示NK细胞的Notch信号与GVHD之间的关系。