Identification of flavonoids-binding proteins, and basic research of cancer prevention based on molecular mechanisms

类黄酮结合蛋白的鉴定及基于分子机制的癌症预防基础研究

• 批准号: 21890223
• 负责人: IIZUMI Yosuke
• 金额: $ 1.7万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21890223/
• 关键词: ケミカルバイオロジー; ナノ磁性ビーズ; フラボノイド; アピゲニン; 癌予防; 標的分子; 作用機序; プロテオミクス

A number of flavonoids in food are believed to have cancer chemopreventive activity. This research identified flavonoids-binding proteins by utilizing nano-magnetic beads and MALDI-TOF MS. Moreover, it was clarified that apigenin-binding protein 1 (ABP1) regulated the G2 phase of the cell cycle, and apigenin inhibited the proliferation of HT-29 cells by inhibiting ABP1. This achievement is the first evidence that explains thecancer chemopreventive activity of flavonoids from flavonoids-binding proteins.

食物中的许多类黄酮被认为具有癌症化学预防活性。该研究利用纳米磁珠和 MALDI-TOF MS 鉴定了类黄酮结合蛋白。此外，还阐明芹菜素结合蛋白1（ABP1）调节细胞周期的G2期，芹菜素通过抑制ABP1来抑制HT-29细胞的增殖。这一成就是解释类黄酮结合蛋白中的类黄酮的癌症化学预防活性的第一个证据。

项目成果

Colloids in Biotechnology(Affinity Magnetic Beads for Chemical Biology and Medicine)Chapter 9

生物技术中的胶体（用于化学生物学和医学的亲和磁珠）第 9 章

• 发表时间: 2011
• 作者: Yosuke Iizumi;Yasuaki Kabe;Mamoru Hatakeyama;Satoshi Sakamoto;Hiroshi Handa
• 通讯作者: Hiroshi Handa

Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway

• DOI: 10.1111/j.1365-2443.2010.01385.x
• 发表时间: 2010-04-01
• 期刊: GENES TO CELLS
• 影响因子: 2.1
• 作者: Kume, Kanako;Iizumi, Yosuke;Handa, Hiroshi
• 通讯作者: Handa, Hiroshi

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