Simulation and analysis of stem cell-carcinogenesis induced by chemical carcinogens using murine embryonic stem cell

在MES细胞和MEF中检测到Cyp1a1和CyP1b1的表达。MEF中细胞色素P1B1的表达程度是MES细胞的14倍。3-甲基胆蒽(3-MC)可诱导两种细胞Cyp1a1和CyP1B1的表达，并呈剂量依赖关系。尤其是在MEF中，Cyp1a1被显著诱导。这些结果表明，疏水性外源物质倾向于集中在未分化的细胞中，如组织干细胞，尽管还需要进一步的研究。

Cyp1a1 and Cyp1b1 were detected in mES cell and MEF. The extent of Cyp1b1 expression was 14 times higher in MEF as compared with mES cell. 3-Methylcholanthrene (3-MC) treatment was induced Cyp1a1 and Cyp1b1 expressions in both cells in a dose-dependent manner. Especially, Cyp1a1 was induced significantly in MEF. These results suggest that hydrophobic xenobiotics tend to be concentrated in undifferentiated cells such as tissue stem cells, although further study is needed.