Identification of a new epitope of Cancer/Testis antigen KK-LC-1 and develop new adoptive immunotherapy

建立了以KK-LC-1肽段为检测靶点的ELISA法测定KK-LC-1抗体滴度的方法。2例患者KK-LC-1抗原抗体滴度高。在这些病例中，我们试图通过反向免疫学方法在HLA-A2或HLA-A24限制的背景下鉴定新的表位，但这是不可能的。因此，利用自体患者的淋巴结淋巴细胞，可以通过肽脉冲的方法将CTL限制性HLA-A2诱导到树突状细胞，并可以鉴定出新的表位。

Method of antibody titer against KK-LC-1 by ELISA using peptide of KK-LC-1 is established. Two patients showed high antibody titer against KK-LC-1 antigen. In these cases, we tried to identify a new epitope in the context of HLA-A2 or HLA-A24 restriction by the Reverse Immunology method, but it was not possible. Therefore, using the lymph node lymphocytes of an autologous patient, the CTL restricted HLA-A2 could be induced by a method of a peptide pulse to dendritic cells, and the new epitope could be identified.