Elucidation of membrane dynamics and molecular mechanism of bone resorption in osteoclast by using multi-photon microscopy

骨通过骨形成和骨吸收之间的平衡来维持。破骨细胞是在我们体内骨吸收中起主要作用的细胞。在这项研究中，为了阐明骨吸收的分子机制，破骨细胞的囊泡运输进行了分析。通过进行4D成像，不仅可以水平地而且可以垂直地分析活破骨细胞中可能参与骨吸收的酸性囊泡的动力学。

Bone is maintained by a balance between bone formation and resorption. The osteoclast is the cell that plays a major role in bone resorption in our body. In this study, to elucidate the molecular mechanism of bone resorption, vesicular trafficking in osteoclast was analyzed. By carrying out 4D-imaging, it becomes possible to analyze dynamics of acidic vesicles, probably involved in bone resorption, in living osteoclast not only horizontally but also vertically.