Imaging of membrane dynamics in specialized differentiated cells by multiphoton super-resolution microscopy

在人体内，稳态是由各种专门分化的细胞的功能来维持的。大部分功能是由于囊泡运输和膜融合。在这项工作中，破骨细胞和脂肪细胞的囊泡贩运进行了分析与多光子荧光4D显微镜。此外，多光子显微镜与我们以前开发的液晶器件的组合实现了高横向分辨率和深景深。

In human body, homeostasis is maintained by functions of various specialized differentiated cells. Most of functions are due to vesicular trafficking and membrane fusion. In this work, vesicular trafficking in osteoclasts and adipocytes was analyzed with multiphoton fluorescent 4D microscopy. Additionally, combination of multiphoton microscopy with our previously developed liquid crystal devices realized both high lateral resolution and deep depth-of-field.