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Biocompatibility and surface modification of metallic biomaterials in biochemical/ mechanical environment

生化/机械环境下金属生物材料的生物相容性和表面改性

基本信息

批准号：
22860036
负责人：
MIYABE Sayaka
金额：
$ 2.01万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Research Activity Start-up
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2011
项目状态：
已结题

项目摘要

In this study, I revealed that dissolution and repassivaiton behavior strongly depended on substrate material even with/ without cells. On Ti6Al4V alloy, a slight increase of current was observed in elastic region due to the breakdown of passive films. Then soon after the plastic deformation started, the current rapidly increased, indicating that the area of newly created surfaces drastically increased as a result of formation of slip band. The similar current behavior was observed on pure Ti. On the other hand, for type 316L stainless steel, the current did not change in elastic region and started to increase at the onset of plastic deformation. After the end of elongation, the current rapidly decreased approaching to the level before straining as a result of repassivation. For the charge density during deformation, there was little difference with/ without cells. On the other hand, for the charge density after deformation, the larger charge density was recognized on the sample adsorbed proteins and the maximum charge density was observed on the sample with cells. This result suggests that proteins and cells inhibit the repassivation. When proteins or cells accumulate on the sample surface, a closed space is made between proteins or cells and metal surface. In this space, the diffusion of solute is blocked and H+ ions reach a high concentration. As a result, the pH becomes low and repassivation is inhibited. The current stagnation on Ti6Al4V alloy with cells is the remarkable example of the repassivation inhibition.

在这项研究中，我发现溶解和repassivaiton行为强烈依赖于基板材料，即使有/没有细胞。在Ti6 Al 4V合金上，由于钝化膜的击穿，在弹性区观察到轻微的电流增加。塑性变形开始后不久，电流迅速增加，表明由于滑移带的形成，新产生的表面面积急剧增加。在纯钛上观察到类似的电流行为。另一方面，对于316L型不锈钢，电流在弹性区域没有变化，并且在塑性变形开始时开始增加。拉伸结束后，由于再钝化，电流迅速下降，接近应变前的水平。对于变形过程中的电荷密度，有/没有细胞的差异很小。另一方面，对于变形后的电荷密度，在吸附蛋白质的样品上识别出较大的电荷密度，并且在具有细胞的样品上观察到最大电荷密度。这一结果表明，蛋白质和细胞抑制再钝化。当蛋白质或细胞聚集在样品表面时，蛋白质或细胞与金属表面之间形成封闭空间。在这个空间中，溶质的扩散被阻止，H+离子达到高浓度。结果，pH变低，再钝化被抑制。Ti6 Al 4V合金的电流滞止现象是再钝化抑制的典型例子。