Functional analysis of small RNA in the reprogramming process from differentiated cells to pluripotent stem cells

我们已经发现，在苔藓Physcomitrella patens中，小RNA的表达在重新编程过程中全局减少。我们假设PpCSP蛋白参与了这一过程。PpCSP被认为在抑制microRNA(MiRNA)成熟方面发挥作用。在这里，我们发现PpCSP定位于胞浆，PpCSP损伤会导致减弱的重编程。这些数据表明，PpCSP通过抑制胞浆中miRNA的成熟来促进重新编程。为了验证这一假设，我们对PpCSP进行了功能分析。

We had found that expression of small RNAs is globally reduced during reprogramming in the moss Physcomitrella patens. We hypothesized that PpCSP proteins are involved in this process. PpCSP is presumed to function in the repression of the microRNA(miRNA) maturation. Here we found that PpCSP was localized in cytosol, and a PpCSP lesion caused attenuated reprogramming. These data suggest that PpCSP facilitates reprogramming through repressing the miRNA maturation in cytosol. To verify this hypothesis, we have performed the functional analyses of PpCSP.