Role of Exol in germ cell apoptosis during spermatogenesis

Exol 在精子发生过程中生殖细胞凋亡中的作用

• 批准号: 22880002
• 负责人: NAMIKI Yuka
• 金额: $ 2.01万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22880002/
• 关键词: 減数分裂; アポトーシス; Exo1

MRL/MpJ mouse testes have metaphase-specific apoptosis(msa) in meiotic spermatocytes. In our previous study, msa occurs due to mutation of the exonuclease 1(Exo1) located on Chr1 of 81. 9cM. In this study, in order to find practical factors in msa,(1) we created B6 background congenic mice(Cg1) carrying msa loci derived from MRL mice.(2) I made purified RNA sample for microarray to know mRNA expression level of apoptosis related genes in testes from Cg1 and B6 mice as a control(6 weeks of age).

MRL/MpJ小鼠睾丸减数分裂精细胞中存在中期特异性凋亡（msa）。在我们之前的研究中，msa的发生是由于位于81号染色体Chr1上的外切酶1（Exo1）的突变。9厘米。在本研究中，为了寻找msa的实际影响因素，(1)我们构建了B6背景基因小鼠（Cg1），携带来自MRL小鼠的msa位点。(2)制备纯化RNA样品进行微阵列检测，以Cg1和B6小鼠（6周龄）为对照，了解睾丸细胞凋亡相关基因mRNA表达水平。