Development of the synthetic method of glycoproteins with homogenous glycan structure oriented toward functional analysis of glycans

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In this study, we tried to develop a novel thiol protecting group which was useful for protecting Cys side chain. As a result, Pocam group could be designed, synthesized and used for solid-phase peptide synthesis and peptide condensation reactions. On the other hand, we tried to synthesize neo-glycoproteins by the combination of bacterial recombinant protein expression system and in vitro glycosylation reaction. As a result, we could prepare a recombinant protein containing a specific functional group. A glycopeptide carrying S-linked glycan which was recently found as a novel post-translational modification was chemically synthesized.