Analysis of ubiquitination pathway to intracytoplasmic bacteria

泛素化ActA缺陷型单核细胞增生李斯特菌（. actA）被自噬识别。为了阐明李斯特菌泛素化的机制，我们试图探索细菌的泛素化分子和宿主的泛素化因子。结果，通过LC-MS/MS分析，鉴定了两个细菌泛素化分子的候选者和四个参与宿主泛素化系统的候选者。通过体外泛素化实验检测了李斯特菌候选分子的重组蛋白，其中一个候选分子在李斯特菌中直接被泛素化。

Ubiquitinated ActA-deficient Listeria monocytogenes(. actA) were recognized by autophagy. To elucidate the mechanism of Listeria ubiquitination, we attempted to explore the bacterial ubiquitinized molecules and the host ubiquitination factors. As a result, two candidates of bacterial ubiquitinated molecules and four candidates involved in host ubiquitination system were identified by LC-MS/MS analysis. The recombinant proteins of Listeria candidate moleculesexamined by in vitro ubiquitination assay, and one of the two candidates inListeria wa weres received ubiquitination directly.