Analysis of ubiquitination pathway to intracytoplasmic bacteria

胞浆内细菌泛素化途径分析

• 批准号: 22890154
• 负责人: YOSHIKAWA Yuko
• 金额: $ 2.01万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22890154/
• 关键词: 細菌; 感染症; リステリア; オートファジー; ユビキチン

Ubiquitinated ActA-deficient Listeria monocytogenes(. actA) were recognized by autophagy. To elucidate the mechanism of Listeria ubiquitination, we attempted to explore the bacterial ubiquitinized molecules and the host ubiquitination factors. As a result, two candidates of bacterial ubiquitinated molecules and four candidates involved in host ubiquitination system were identified by LC-MS/MS analysis. The recombinant proteins of Listeria candidate moleculesexamined by in vitro ubiquitination assay, and one of the two candidates inListeria wa weres received ubiquitination directly.

泛素化ActA缺陷型单核细胞增生李斯特菌（. actA）被自噬识别。为了阐明李斯特菌泛素化的机制，我们试图探索细菌的泛素化分子和宿主的泛素化因子。结果，通过LC-MS/MS分析，鉴定了两个细菌泛素化分子的候选者和四个参与宿主泛素化系统的候选者。通过体外泛素化实验检测了李斯特菌候选分子的重组蛋白，其中一个候选分子在李斯特菌中直接被泛素化。

项目成果

A Tecpr1-Dependent Selective Autophagy Pathway Targets Bacterial Pathogens

• DOI: 10.1016/j.chom.2011.04.010
• 发表时间: 2011-05-19
• 期刊: CELL HOST & MICROBE
• 影响因子: 30.3
• 作者: Ogawa, Michinaga;yoshikawa, Yuko;Sasakawa, Chihiro
• 通讯作者: Sasakawa, Chihiro

Evaluation of Wasabi-duke (sake lees containing wasabi) as human probiotics

芥末公爵（含有芥末的酒糟）作为人类益生菌的评价

• 发表时间: 2011
• 作者: 刀坂泰史;村井範之;砂川陽一;川口正悟;矢吹晴一郎;和田啓道;木村剛;藤田正俊;長谷川浩二;森本達也;Yamazaki M.
• 通讯作者: Yamazaki M.

Human infection with Anaplasma phagocytophilum in Japan

日本人类感染嗜吞噬细胞无形体

• 发表时间: 2011
• 作者: 高娃;他
• 通讯作者: 他

Molecular detection of Rickettsiales bacteria from ticks in western Japan

日本西部蜱虫中立克次体细菌的分子检测

• 发表时间: 2011
• 作者: Gaowa;Wuritu;Aochi M.;Kawamori F.;Yoshikawa Y. and Ohashi N
• 通讯作者: Yoshikawa Y. and Ohashi N

Molecular epidemiological study of Rickettsiales bacteria in wild deer and mice in Shizuoka prefecture, Japan

日本静冈县野鹿和小鼠立克次氏体细菌的分子流行病学研究

• 发表时间: 2011
• 作者: Wuritu;Wu D.;Yoshikawa Y.;Furukawa E.;Murakami T.;Minato C.;Kawamori F.;Ohtake M.;Ohashi M. and Ohashi N
• 通讯作者: Ohashi M. and Ohashi N