Controlling the higher order structure of single DNA molecule with attention to environmental stress

关注环境胁迫控制单个DNA分子的高阶结构

• 批准号: 22510123
• 负责人: YOSHIKAWA Yuko
• 金额: $ 2.41万
• 依托单位: Ritsumeikan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22510123/
• 关键词: 1分子計測; 環境応答; 長鎖DNA; DNA損傷; 蛍光顕微鏡; 単一分子観察; DNAの高次構造; 抗がん剤; 抗酸化物質

1. The protective effects of water-soluble derivatives of flavonoids on photo- and gamma ray-induced DNA double-strand breaks were evaluated in a quantitative manner. This observation demonstrated the dependence of the antioxidant potentials of these compounds on the source of irradiation. 2. It was found that tetrazolato-bridged dinuclear platinum(II) complexes induced the irreversible compaction of large genomic DNA, which was accompanied by a change in the secondary structure from the B to C form. 3.The appearance of the phase-segregated state in megabase-size DNA is markedly different from the all-or-none characteristics on the foldingtransition in a single sub-megabase-size DNA. 4. Novel stereoisomers of tetravalent polyamines were examined their ability to induce the compaction of single DNA molecules. It was found that Isomer (R,S-R,S) is more potent at inducing both DNA compaction and changes in the secondary structure.

1.定量评价了黄酮类化合物对光、伽马射线诱导的DNA双链断裂的保护作用。这一观察证明了这些化合物的抗氧化潜力与辐射来源的依赖关系。2.研究发现，四唑桥联双核铂(II)配合物诱导了大基因组DNA的不可逆致密化，并伴随着二级结构从B型向C型的转变。3.兆碱基DNA的相分离状态与单一亚兆碱基DNA的折叠转变的全有或全无特征明显不同。4.考察了新型四价多胺立体异构体对DNA单分子的致密化作用。研究发现，异构体(R、S-R、S)对DNA的致密化和二级结构的改变均有较强的诱导作用。

项目成果

Direct observation of large-scale conformational transition in megabase-size DNA

直接观察兆碱基 DNA 中的大规模构象转变

• 发表时间: 2011
• 作者: Kazuki Kojima;Wilson Agerico Dino;Masahiko Suzuki;Tsuneo Yasue;Kazue Kudo;Noriko Akutsu;Ernst Bauer;Takanori Koshikawa,Hideaki Kasai;Yuko Yoshikawa;Adhitya G. Saputro,Hideaki Kasai;伴雅人;Yuko Yoshikawa
• 通讯作者: Yuko Yoshikawa

Critical behavior of megabase-size DNA toward the transition into a compact state

兆碱基大小 DNA 向致密状态转变的关键行为

• DOI: 10.1063/1.3666845
• 发表时间: 2011
• 期刊: Journal of Chemical Physics
• 影响因子: 4.4
• 作者: Yuko Yoshikawa;Yuki Suzuki;Kozo Yamada;Wakao Fukuda;Kenichi Yoshikawa;Kunio Takeyasu;Tadayuki Imanaka
• 通讯作者: Tadayuki Imanaka

Single molecule real-time observation of DNA interacting with dinuclear platinum antitumordrugs

单分子实时观察DNA与双核铂类抗肿瘤药物相互作用

• 发表时间: 2012
• 作者: Koji Shimizu;W.A. Dino;Hideaki Kasai;Yuko Yoshikawa
• 通讯作者: Yuko Yoshikawa

Comparative study of kinetics on DNA double-strand break induced by photo- and gamma-irradiation: Protective effect of water-soluble flavonoids

• DOI: 10.1016/j.cplett.2010.11.003
• 发表时间: 2010-12-06
• 期刊: CHEMICAL PHYSICS LETTERS
• 影响因子: 2.8
• 作者: Yoshikawa, Yuko;Mori, Toshiaki;Yoshikawa, Kenichi
• 通讯作者: Yoshikawa, Kenichi

Direct observation of large-scale conformational transitionin megabase-size DNA

直接观察兆碱基 DNA 中的大规模构象转变

• 发表时间: 2011
• 作者: Kazuki Kojima;Wilson Agerico Dino;Masahiko Suzuki;Tsuneo Yasue;Kazue Kudo;Noriko Akutsu;Ernst Bauer;Takanori Koshikawa,Hideaki Kasai;Yuko Yoshikawa
• 通讯作者: Yuko Yoshikawa