海洋微生物光受容の全貌解明：大規模メタゲノムで描き出す海と光のSeascape

阐明海洋微生物感光的全貌：利用大规模宏基因组描绘海洋和光的海景

• 批准号: 22H00557
• 负责人: 吉澤 晋
• 金额: $ 26.12万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22H00557/
• 关键词: 海洋微生物; 微生物生態; 光受容体; メタゲノム

本研究では、これまでに実施されたほぼ全ての海洋メタゲノムプロジェクトを集約・再アセンブル・再解析を行い、海洋微生物を全球レベルで比較可能なデータベースを構築する。構築したデータベースを用いて、海洋微生物の持つ光受容システム（ロドプシンや光合成に関連する遺伝子）の分布を網羅的に解析する。また、メタゲノム再構築ゲノム（MAG）から、各遺伝子を持つ生物種の特定を行い、上記解析結果と統合することで、海洋微生物の持つ光受容システムを全球レベルで可視化することを目指す。メタゲノムデータを用いて、個々の微生物ゲノム（MAG：Metagenome-Assembled Genome）を解読する手法の開発をおこない、開発した手法を用いて、公共データベースから入手可能な、様々な海洋環境に由来する大規模メタゲノムデータ（約29兆塩基対）の解析を行なった。その結果、59門にまたがる5万以上の原核生物ゲノムの解読に成功し、得られたゲノムの情報を整備して、約15,000ゲノムを収載する海洋微生物ゲノムデータベースMarDBを大幅に上回る、世界最大の海洋微生物ゲノムカタログ「OceanDNA MAGカタログ」として公開した。構築した世界最大の微生物ゲノムデータベースを用いることで、これまでにない解像度で機能遺伝子の分布および遺伝子を持つ分類群を調べることが可能になる。今後の研究では、データベースを用いて、微生物型ロドプシン、光合成関連遺伝子（pufM）の全球分布の解析を実施予定である。

In this study, the results of this study are as follows: (1) in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: 1. In this paper, we use the analysis of the distribution network of light tolerance (photosynthesis) and marine microorganisms. In this paper, the results of the previous analysis show that the results of the previous analysis are in accordance with the results of the previous analysis, and the results of the previous analysis are consistent with the results of the previous analysis. the results of the previous analysis show that the results of the previous analysis are consistent with the results of the previous analysis, and that the marine microorganisms are sensitive to light tolerance. You can use microbiological information technology (MAG:Metagenome-Assembled Genome) to analyze the nature of the ocean environment, and to analyze the nature of the marine environment. The results showed that more than 59 million samples of prokaryotes were successfully resolved, and about 15000 of them were successfully reported. The results show that marine microorganisms are the largest in the world, and the world's largest marine microbes are known as "OceanDNA MAG microbes". In the world's largest microbiological system, the system is used to measure the resolution of the system. The system is capable of monitoring the distribution of sub-data. In the future, we will study and analyze the global distribution of microbes, microorganisms, and photosynthesis (pufM).

项目成果

Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers.

• DOI: 10.1073/pnas.2117433119
• 发表时间: 2022-03-01
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Hosaka T;Nomura T;Kubo M;Nakane T;Fangjia L;Sekine SI;Ito T;Murayama K;Ihara K;Ehara H;Kashiwagi K;Katsura K;Akasaka R;Hisano T;Tanaka T;Tanaka R;Arima T;Yamashita A;Sugahara M;Naitow H;Matsuura Y;Yoshizawa S;Tono K;Owada S;Nureki O;Kimura-Someya T;Iwata S;Nango E;Shirouzu M
• 通讯作者: Shirouzu M

「微生物ダークマター」を解き明かす ―世界最大の海洋微生物ゲノムカタログ―

解开“微生物暗物质”——世界上最大的海洋微生物基因组目录——

The OceanDNA MAG Catalog: an Unprecedented-scale Genome Resource of Marine Prokaryotes

OceanDNA MAG 目录：规模空前的海洋原核生物基因组资源

• 发表时间: 2022
• 作者: Nishimura Yosuke;Yoshizawa Susumu;和田茂樹;Yosuke Nishimura and Susumu Yoshizawa
• 通讯作者: Yosuke Nishimura and Susumu Yoshizawa

Does proteorhodopsin have any antenna mechanism?

变形视紫红质有天线机制吗？

• 发表时间: 2022
• 作者: Takayoshi Fujiwara;Masumi Hasegawa;Keiichi Inoue;Susumu Yoshizawa
• 通讯作者: Susumu Yoshizawa

Light-utilization strategies with microbial rhodopsins in cyanobacteria

蓝细菌中微生物视紫红质的光利用策略

• 发表时间: 2022
• 作者: Masumi Hasegawa;Toshiaki Hosaka;Keiichi Kojima;Yosuke Nishimura;Marie Kurihara;Yu Nakajima;Tomomi Kimura-Someya;Mikako Shirouzu;Yuki Sudo;Susumu Yoshizawa
• 通讯作者: Susumu Yoshizawa