虚血性心筋を標的とするナノボディ修飾細胞外小胞を使用した心筋梗塞治療法の開発

我们已经构建了一种新型的模块化合成纳米体噬菌体展示文库，用于筛选CD8、PD-1和HER2等靶标。我们还用液相色谱质谱鉴定了常氧和低氧心肌细胞的差异蛋白质。在这些差异蛋白中，CD29和CD166差异最显著，也参与了细胞在低氧条件下的生存。将选择CD29和CD166进行进一步的实验。

We have generated a novel modular synthetic nanobody phage display library to select for targets including CD8, PD-1 and HER2. We also identified differential proteins of normoxic and hypoxic cardiomyocytes by liquid chromatography mass spectrometry. Among these differential proteins, CD29 and CD166 were identified to have most significant difference and also be involved in cell survival in hypoxic conditions. CD29 and CD166 will be chosen for further experiments.