虚血性心筋を標的とするナノボディ修飾細胞外小胞を使用した心筋梗塞治療法の開発

使用纳米抗体修饰的细胞外囊泡靶向缺血心肌开发心肌梗死治疗

• 批准号: 22K20643
• 负责人: ヤン ジュンジー
• 金额: $ 1.83万
• 依托单位: Shonan Kamakura General Hospital, Medical Corporation Tokushukai (Center for Clinical and
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-08-31 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K20643/
• 关键词: nanobody phage library; biopanning; hypoxia; cell engineering; ischemic heart targeted; mesenchymal stem cells; exosomes; Extracellular Vesicles; Mesenchymal Stem Cells; Myocardial Infarction; Regeneration; Nanobody Phage Library

We have generated a novel modular synthetic nanobody phage display library to select for targets including CD8, PD-1 and HER2. We also identified differential proteins of normoxic and hypoxic cardiomyocytes by liquid chromatography mass spectrometry. Among these differential proteins, CD29 and CD166 were identified to have most significant difference and also be involved in cell survival in hypoxic conditions. CD29 and CD166 will be chosen for further experiments.

我们已经产生了一种新的模块化合成纳米抗体噬菌体展示文库，以选择包括CD 8，PD-1和HER 2在内的靶标。我们还通过液相色谱质谱鉴定了常氧和缺氧心肌细胞的差异蛋白质。在这些差异蛋白中，CD 29和CD 166被鉴定为具有最显著的差异，并且也参与了细胞在缺氧条件下的存活。将选择CD 29和CD 166用于进一步的实验。

项目成果

University of Alabama at Birmingham(米国)

阿拉巴马大学伯明翰分校（美国）